Viral replicase gene products suffice for coronavirus discontinuous transcription

被引：119

作者：

Thiel, V ^{[1
]}

Herold, J ^{[1
]}

Schelle, B ^{[1
]}

Siddell, SG ^{[1
]}

机构：

[1] Univ Wurzburg, Inst Immunol & Virol, D-97078 Wurzburg, Germany

来源：

JOURNAL OF VIROLOGY | 2001年 / 75卷 / 14期

关键词：

D O I：

10.1128/JVI.75.14.6676-6681.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have used vaccinia virus as a vector to clone a 22.5-kbp cDNA that represents the 5' and 3' ends of the human coronavirus 229E (HCoV 229E) genome, the HCoV 229E replicase gene, and a single reporter gene (coding for green fluorescent protein [GFP]) located downstream of a regulatory element for coronavirus mRNA transcription. When RNA transcribed from this cDNA was transfected into BHK-21 cells, a small percentage of cells displayed strong fluorescence. A region of the mRNA encoding GFP was amplified by PCR and shown to have the unique mRNA leader-body junction indicative of coronavirus-mediated transcription. These data show that the coronavirus replicase gene products suffice for discontinuous subgenomic mRNA transcription.

引用

页码：6676 / 6681

页数：6

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