Zeaxanthin radical cation formation in minor light-harvesting complexes of higher plant antenna

被引:178
作者
Avenson, Thomas J. [1 ,3 ]
Ahn, Tae Kyu [1 ,2 ]
Zigmantas, Donatas [2 ,3 ]
Niyogi, Krishna K. [2 ,3 ]
Li, Zhirong [2 ,3 ]
Ballottari, Matteo [4 ]
Bassi, Roberto [4 ]
Fleming, Graham R. [1 ,2 ]
机构
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[2] Lawrence Berkeley Natl Lab, Phys Biosci Div, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
[4] Univ Verona, Dept Sci & TEchnol, I-37134 Verona, Italy
关键词
D O I
10.1074/jbc.M705645200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previous work on intact thylakoid membranes showed that transient formation of a zeaxanthin radical cation was correlated with regulation of photosynthetic light-harvesting via energy-dependent quenching. A molecular mechanism for such quenching was proposed to involve charge transfer within a chlorophyll-zeaxanthin heterodimer. Using near infrared (880 - 1100 nm) transient absorption spectroscopy, we demonstrate that carotenoid (mainly zeaxanthin) radical cation generation occurs solely in isolated minor light-harvesting complexes that bind zeaxanthin, consistent with the engagement of charge transfer quenching therein. We estimated that less than 0.5% of the isolated minor complexes undergo charge transfer quenching in vitro, whereas the fraction of minor complexes estimated to be engaged in charge transfer quenching in isolated thylakoids was more than 80 times higher. We conclude that minor complexes which bind zeaxanthin are sites of charge transfer quenching in vivo and that they can assume Non-quenching and Quenching conformations, the equilibrium LHC(N) reversible arrow LHC(Q) of which is modulated by the transthylakoid pH gradient, the PsbS protein, and protein-protein interactions.
引用
收藏
页码:3550 / 3558
页数:9
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