Stimulation effect of galanin-like peptide (GALP) on luteinizing hormone-releasing hormone-mediated luteinizing hormone (LH) secretion in male rats

被引:81
作者
Matsumoto, H [1 ]
Noguchi, J [1 ]
Takatsu, Y [1 ]
Horikoshi, Y [1 ]
Kumano, S [1 ]
Ohtaki, T [1 ]
Kitada, C [1 ]
Itoh, T [1 ]
Onda, H [1 ]
Nishimura, O [1 ]
Fujino, M [1 ]
机构
[1] Takeda Chem Ind Ltd, Pharmaceut Discovery Res Div, Discovery Res Labs 1, Tsukuba, Ibaraki 3004293, Japan
关键词
D O I
10.1210/en.142.8.3693
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Galanin-like peptide (GALP) is a recently isolated hypothalamic peptide which has sequence homology to galanin and binds to galanin receptors with high affinity. It has been shown that GALP neurons are localized in the arcuate nucleus and that GALP-immunoreactive fibers are in close apposition with LHRH neurons in the medial preoptic area (MPA). In the present study, we found that intracerebroventricular (icv) administration of GALP increased the plasma LH level but did not change the levels of other hormones. Concomitantly, accumulation of c-Fos protein was dramatically increased in the nuclei of LHRH-positive cells in the MPA by icv GALP administration. Furthermore, the GALP-induced plasma LH response was completely abolished by pretreatment with Cetrorelix, a LHRH receptor antagonist. On the other hand, GALP did not affect the release of LH, FSH, TSH, ACTH, GH or PRL directly from dispersed rat pituitary cells in vitro. These results strongly suggest a role for GALP in the control of gonadotropin secretion through a hypothalamic mechanism involving the release of LHRH.
引用
收藏
页码:3693 / 3696
页数:4
相关论文
共 15 条
[1]   Influence of endogenous leptin tone on the estrous cycle and luteinizing hormone pulsatility in female rats [J].
Carro, E ;
Pinilla, L ;
Seoane, LM ;
Considine, RV ;
Aguilar, E ;
Casanueva, FF ;
Dieguez, C .
NEUROENDOCRINOLOGY, 1997, 66 (06) :375-377
[2]   Distribution, regulation and role of hypothalamic galanin systems: Renewed interest in a pleiotropic peptide family [J].
Gundlach, AL ;
Burazin, TCD ;
Larm, JA .
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2001, 28 (1-2) :100-105
[3]  
ITO T, 2000, J CHEM SOC P1, P1333
[4]   Galanin-like peptide (GALP) is a target for regulation by leptin in the hypothalamus of the rat [J].
Juréus, A ;
Cunningham, MJ ;
McClain, ME ;
Clifton, DK ;
Steiner, RA .
ENDOCRINOLOGY, 2000, 141 (07) :2703-2706
[5]   Analyses for susceptibility of rat anterior pituitary cells to prolactin-releasing peptide [J].
Kawamata, Y ;
Fujii, R ;
Fukusumi, S ;
Habata, Y ;
Hosoya, M ;
Hinuma, S ;
Kitada, C ;
Onda, H ;
Nishimura, O ;
Fujino, M .
ENDOCRINE, 2000, 12 (03) :215-221
[6]   3RD-VENTRICULAR INFUSION OF NEUROPEPTIDE-Y SUPPRESSES LUTEINIZING-HORMONE SECRETION IN OVARIECTOMIZED RHESUS MACAQUES [J].
KAYNARD, AH ;
PAU, KYF ;
HESS, DL ;
SPIES, HG .
ENDOCRINOLOGY, 1990, 127 (05) :2437-2444
[7]   Galanin-like peptide (GALP) is expressed in rat hypothalamus and pituitary, but not in DRG [J].
Kerr, NCH ;
Holmes, FE ;
Wynick, D .
NEUROREPORT, 2000, 11 (17) :3909-3913
[8]   Galanin-like peptide (GALP) mRNA expression is restricted to arcuate nucleus of hypothalamus in adult male rat brain [J].
Larm, JA ;
Gundlach, AL .
NEUROENDOCRINOLOGY, 2000, 72 (02) :67-71
[9]   Morphological evidence for direct interaction between arcuate nucleus neuropeptide Y (NPY) neurons and gonadotropin-releasing hormone neurons and the possible involvement of NPYY1 receptors [J].
Li, C ;
Chen, PL ;
Smith, MS .
ENDOCRINOLOGY, 1999, 140 (11) :5382-5390
[10]   Isolation and cDNA cloning of a novel galanin-like peptide (GALP) from porcine hypothalamus [J].
Ohtaki, T ;
Kumano, S ;
Ishibashi, Y ;
Ogi, K ;
Matsui, H ;
Harada, M ;
Kitada, C ;
Kurokawa, T ;
Onda, H ;
Fujino, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (52) :37041-37045