Targeted mutagenesis of acyl-lipid desaturases in Synechocystis: Evidence for the important roles of polyunsaturated membrane lipids in growth, respiration and photosynthesis

被引:186
作者
Tasaka, Y
Gombos, Z
Nishiyama, Y
Mohanty, P
Ohba, T
Ohki, K
Murata, N
机构
[1] NATL INST BASIC BIOL, DEPT REGULAT BIOL, OKAZAKI, AICHI 444, JAPAN
[2] HUNGARIAN ACAD SCI, BIOL RES CTR, INST PLANT BIOL, H-6701 SZEGED, HUNGARY
[3] TOHOKU UNIV, GRAD SCH SCI, DEPT PHYS, AOBA KU, SENDAI, MIYAGI 98077, JAPAN
关键词
acyl-lipid desaturase; membrane glycerolipid; photoinhibition; Synechocystis sp PCC 6803;
D O I
10.1002/j.1460-2075.1996.tb01033.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Acyl-lipid desaturases introduce double bonds (unsaturated bonds) at specifically defined positions in fatty acids that are esterified to the glycerol backbone of membrane glycerolipids. The desA, desB and desD genes of Synechocystis sp. PCC 6803 encode acyl-lipid desaturases that introduce double bonds at the Delta 12, omega 3 and Delta 6 positions of C-18 fatty acids respectively. The mutation of each of these genes by insertion of an antibiotic resistance gene cartridge completely eliminated the corresponding desaturation reaction. This system allowed us to manipulate the number of unsaturated bonds in membrane glycerolipids in this organism in a step-wise manner. Comparisons of the variously mutated cells revealed that the replacement of all polyunsaturated fatty acids by a monounsaturated fatty acid suppressed growth of the cells at low temperature and, moreover, it decreased the tolerance of the cells to photoinhibition of photosynthesis at low temperature by suppressing recovery of the photosystem II protein complex from photoinhibitory damage, However, the replacement of tri- and tetraunsaturated fatty acids by a diunsaturated fatty acid did not have such effects, These findings indicate that polyunsaturated fatty acids are important in protecting the photosynthetic machinery from photoinhibition at low temperatures.
引用
收藏
页码:6416 / 6425
页数:10
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