Cloning and expression of the Zymomonas mobilis ''production of ethanol'' genes in Lactobacillus casei

被引：36

作者：

Gold, RS ^{[1
]}

Meagher, MM ^{[1
]}

Tong, SX ^{[1
]}

Hutkins, RW ^{[1
]}

Conway, T ^{[1
]}

机构：

[1] UNIV NEBRASKA,DEPT FOOD SCI & TECHNOL,LINCOLN,NE 68583

来源：

CURRENT MICROBIOLOGY | 1996年 / 33卷 / 04期

关键词：

D O I：

10.1007/s002849900109

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study describes the expression of the Zymomonas mobilis genes coding for pyruvate decarboxylase (pdc) and alcohol dehydrogenase (adh) in Lactobacillus casei 686. To promote transcription, the promoter and ribosome binding site (RES) from the Lactococcus lactis subsp. lactis-derived vector, pMGE36e, were inserted upstream of the pde gene, The former sequences were positioned such that translation of pdc was coupled to translation of an 81-base pair open reading frame terminating within the pde initiation site. The recombinant plasmid (pRSG02) was electroporated into L. casei, and transformants were obtained. Northern analysis confirmed the production of a 3.1-kb transcript corresponding to the predicted size of the PET operon. Western blot analyses revealed that the recombinant strain expressed both enzymes. The recombinant produced more than twice the ethanol produced by the parental L. casei strain.

引用

页码：256 / 260

页数：5

共 24 条

[1] TRANSFORMATION OF LACTOBACILLUS-REUTERI WITH ELECTROPORATION - STUDIES ON THE ERYTHROMYCIN RESISTANCE PLASMID PLUL631 [J].