Multiplex polymerase chain reaction assay for identification of enterotoxigenic Escherichia coli strains

被引：40

作者：

Osek, J ^{[1
]}

机构：

[1] Natl Inst Vet Res, Dept Microbiol, PL-24100 Pulawy, Poland

来源：

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION | 2001年 / 13卷 / 04期

关键词：

D O I：

10.1177/104063870101300405

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A multiplex polymerase chain reaction (PCR) system was developed for identification of enterotoxiogenic Escherichia coli (ETEC) strains and to differentiate them from other gram negative enteric bacteria. This test simultaneously amplifies heat-labile (LTI) and heat-stable (STI and STII) toxin sequences and the E. coli-specific universal stress protein (uspA). The specificity of the method was validated by single PCR tests performed with the reference E. coli and non-E. coli strains and with bacteria isolated from pig feces. The multiplex PCR allowed the rapid and specific identification of enterotoxin-positive E. coli and may be used as a method for direct determination of ETEC and to differentiate them from other E. coli and gram-negative enteric isolates.

引用

页码：308 / 311

页数：4

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