Development of a novel screening method for the isolation of "Cronobacter" spp. (Enterobacter sakazakii)

被引:63
作者
Iversen, Carol [1 ,2 ]
Druggan, Patrick [3 ]
Schumacher, Sandra [1 ]
Lehner, Angelika [1 ]
Feer, Claudia [4 ]
Gschwend, Karl [4 ]
Joosten, Han
Stephan, Roger [1 ]
机构
[1] Univ Zurich, Vetsuisse Fac, Inst Food Safety & Hygiene, CH-8057 Zurich, Switzerland
[2] Nestle Res Ctr, Qual & Safety Dept, CH-1000 Lausanne, Switzerland
[3] Oxoid Ltd, Thermo Fisher Sci, Basingstoke RG24 8PW, Hants, England
[4] Hochdorf Nutritec AG, Food Safety Dept, CH-6280 Hochdorf, Switzerland
关键词
D O I
10.1128/AEM.02801-07
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A differential medium, "Cronobacter" screening broth, has been designed to complement agars based on hydrolysis of chromogenic alpha-glucopyranoside substrates. The broth was evaluated using 329 Enterobacteriaceae strains (229 target isolates), spiked/naturally contaminated samples, and a parallel comparison with current methods for raw materials, line/end products, and factory environment samples.
引用
收藏
页码:2550 / 2553
页数:4
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