Chlorination and ozonation of waste-water:: comparative analysis of efficacy through the effect on Escherichia coli membranes

被引:46
作者
Arana, I [1 ]
Santorum, P [1 ]
Muela, A [1 ]
Barcina, I [1 ]
机构
[1] Univ Basque Country, Fac Ciencias, Dept Inmunol Microbiol & Parasitol, E-48080 Bilbao, Spain
关键词
D O I
10.1046/j.1365-2672.1999.00772.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The effect of chlorine and ozone on Escherichia coli cells resuspended in waste-water was compared. Selected chlorination and ozonation conditions produced a similar decrease in culturability (2-2.5 log). Under these conditions, differences in membrane permeability and cell surface hydrophobicity, depending on the disinfectant tested, were detected. After ozonation, while no changes in cell surface hydrophobicity were observed, approximately 95.5% of cells showed altered membrane permeability. The effect of chlorine was not linked to changes in membrane permeability. After chlorination, E. coli cells showed a tendancy to aggregate. The possibility that aggregation of cells could interfere with conventional colony counts is discussed. The degree of toxicity (Microtox(TM) assay) was unrelated to the effect on cellular activity.
引用
收藏
页码:883 / 888
页数:6
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