Genetic diversity in the lactose operons of Lactobacillus helveticus strains and its relationship to the role of these strains as commercial starter cultures

被引:16
作者
Callanan, MJ [1 ]
Beresford, TP [1 ]
Ross, RP [1 ]
机构
[1] TEAGASC, Dairy Prod Res Ctr, Cork, Ireland
关键词
D O I
10.1128/AEM.71.3.1655-1658.2005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Two novel insertion sequence elements, ISLhe1 and ISLhe15, were located upstream of the genes encoding the P-galactosidase enzyme in Lactobacillas helveticus commercial starter strains. Strains with the IS982 family element, ISLhe1, demonstrated reduced beta-galactosidase activity compared to the L. helveticus type strain, whereas strains with the ISLhe15 element expressed beta-galactosidase in the absence of lactose.
引用
收藏
页码:1655 / 1658
页数:4
相关论文
共 12 条
[1]   Application of the polymerase chain reaction to the rapid analysis of brewery yeast strains [J].
Coakley, M ;
Ross, RP ;
Donnelly, D .
JOURNAL OF THE INSTITUTE OF BREWING, 1996, 102 (05) :349-354
[2]  
Cogan TM, 1996, Dairy Starter Culture, P1
[3]  
DE MAN J. C., 1960, JOUR APPL BACT, V23, P130, DOI 10.1111/j.1365-2672.1960.tb00188.x
[4]   Transcriptional activation of the citrate permease P gene of Lactococcus lactis biovar diacetylactis by an insertion sequence like element present in plasmid pCIT264 [J].
deFelipe, FL ;
Magni, C ;
deMendoza, D ;
Lopez, P .
MOLECULAR AND GENERAL GENETICS, 1996, 250 (04) :428-436
[5]   Unusual organization for lactose and galactose gene clusters in Lactobacillus helveticus [J].
Fortina, MG ;
Ricci, G ;
Mora, D ;
Guglielmetti, S ;
Manachini, PL .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (06) :3238-3243
[6]  
FROHMAN MA, 1994, PCR METH APPL, V4, P40
[7]   Use of autolytic starter systems to accelerate the ripening of Cheddar cheese [J].
Hannon, JA ;
Wilkinson, MG ;
Delahunty, CM ;
Wallace, JM ;
Morrissey, PA ;
Beresford, TP .
INTERNATIONAL DAIRY JOURNAL, 2003, 13 (04) :313-323
[8]  
Kiernan RC, 2000, IRISH J AGR FOOD RES, V39, P95
[9]   Regulation and adaptive evolution of lactose operon expression in Lactobacillus delbrueckii [J].
Lapierre, L ;
Mollet, B ;
Germond, JE .
JOURNAL OF BACTERIOLOGY, 2002, 184 (04) :928-935
[10]   Insertion sequences [J].
Mahillon, J ;
Chandler, M .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 1998, 62 (03) :725-+