Preparation of N-succinimidyl 3-[*I] iodobenzoate:: an agent for the indirect radioiodination of proteins

被引：51

作者：

Vaidyanathan, Ganesan ^{[1
]}

Zalutsky, Michael R. ^{[1
]}

机构：

[1] Duke Univ, Med Ctr, Dept Radiol, Durham, NC 27710 USA

来源：

NATURE PROTOCOLS | 2006年 / 1卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nprot.2006.99

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for the synthesis of N-succinimidyl 3-iodobenzoate labeled with any iodine isotope ([* I] SIB), which is an agent used in the radioiodination of proteins and peptides, from its tin precursor N-succinimidyl 3-(tri-n-butylstannyl) benzoate (STB) is described. Also included are protocols for the synthesis of an unlabeled standard of SIB and the tin precursor. Radioiododestannylation of STB using tert-butylhydroperoxide as the oxidant gives [* I] SIB in 80% radiochemical yields. The total time for the synthesis of [* I] SIB from STB is similar to 95 min. Use of [* I] SIB yields radioiodinated proteins that are considerably more stable in vivo than those radioiodinated by the direct electrophilic method.

引用

页码：707 / 713

页数：7

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