The ratio of campesterol to sitosterol that modulates growth in Arabidopsis is controlled by STEROL METHYLTRANSFERASE 2;1

被引：137

作者：

Schaeffer, A ^{[1
]}

Bronner, R ^{[1
]}

Benveniste, P ^{[1
]}

Schaller, H ^{[1
]}

机构：

[1] Inst Bot, Dept Biosynth & Fonct Isoprenoides, CNRS, Inst Biol Mol Plantes, F-67083 Strasbourg, France

来源：

PLANT JOURNAL | 2001年 / 25卷 / 06期

关键词：

Arabidopsis; sterol-C24-methyltransferases; campesterol; sitosterol; brassinosteroids; growth; development;

D O I：

10.1046/j.1365-313x.2001.00994.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The Arabidopsis genome contains three distinct genes encoding sterol-C24-methyltransferases (SMTs) involved in sterol biosynthesis. The expression of one of them, STEROL METHYLTRANSFERASE 2;1, was modulated in 35S::SMT2;1 Arabidopsis in order to study its physiological function. Plants overexpressing the transgene accumulate sitosterol, a 24-ethylsterol which is thought to be the typical plant membrane reinforcer, at the expense of campesterol. These plants displayed a reduced stature and growth that could be restored by brassinosteroid treatment. Plants showing co-suppression of SMT2;1 were characterized by a predominant 24-methylsterol biosynthetic pathway leading to a high campesterol content and a depletion in sitosterol. Pleiotropic effects on development such as reduced growth, increased branching, and low fertility of high-campesterol plants were not modified by exogenous brassinosteroids, indicating specific sterol requirements to promote normal development. Thus SMT2;? has a crucial role in balancing the ratio of campesterol to sitosterol in order to fit both growth requirements and membrane integrity.

引用

页码：605 / 615

页数：11

共 47 条

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