Cytochrome c release from mitochondria is essential for apoptosis. Using human myelogenous leukemia ML-la, its respiration-deficient and reconstituted cells, we demonstrated that respiratory function is essential for tumor necrosis factor-induced cytochrome c release. In a cell free system using mitochondrial fraction from ML-Ia, initiation of respiration by substrates for complexes I, II, and III but not IV released cytochrome c, suggesting that reduction of coenzyme Q or complex III is essential for cytochrome c release. In the same system, disruption of mitochondrial outer membrane was neither enough nor the cause for cytochrome c release by succinate. These observations define an early pathway in which a change in respiration releases cytochrome c. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
机构:
La Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USALa Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USA
Bossy-Wetzel, E
Newmeyer, DD
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La Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USALa Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USA
Newmeyer, DD
Green, DR
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La Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USALa Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USA
机构:
La Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USALa Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USA
Bossy-Wetzel, E
Newmeyer, DD
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机构:
La Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USALa Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USA
Newmeyer, DD
Green, DR
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La Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USALa Jolla Inst Allergy & Immunol, Div Cellular Immunol, San Diego, CA 92121 USA