Isolation and characterization of photosystem II subcomplexes from cyanobacteria lacking photosystem I

被引：4

作者：

Szabò, I

Rigoni, F

Bianchetti, M

Carbonera, D

Pierantoni, F

Seraglia, R

Segalla, A

Giacometti, GM

机构：

[1] Univ Tuscia, Dept Environm Sci, Viterbo, Italy

[2] Univ Padua, Dept Phys Chem, I-35100 Padua, Italy

[3] CNR Res Area, Padua, Italy

[4] Univ Tuscia, Dept Biol, Viterbo, Italy

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2001年 / 268卷 / 19期

关键词：

CP47-RC subcomplex; cyanobacteria; photosystem II;

D O I：

10.1046/j.0014-2956.2001.02441.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A photosystem. II (PSII) core complex lacking the internal antenna CP43 protein was isolated from the photosystem II of Synechocystis PCC6803, which lacks photosystem I (PSI). CP47-RC and reaction centre (RCII) complexes were also obtained in a single procedure by direct solubilization of whole thylakoid membranes. The CP47-RC subcore complex was characterized by SDS/PAGE, immunoblotting, MALDI MS, visible and fluorescence spectroscopy, and absorption detected magnetic resonance. The purity and functionality of RCII was also assayed. These preparations may be useful for mutational analysis of PSII RC and CP47-RC in studying primary reactions of oxygenic photosynthesis.

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页码：5129 / 5134

页数：6

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