POMT2, a key enzyme in Walker-Warburg syndrome:: somatic sPOMT2, but not testis-specific tPOMT2, is crucial for mannosyltransferase activity in vivo

被引:14
作者
Lommel, Mark [1 ]
Willer, Tobias [1 ]
Strahl, Sabine [1 ]
机构
[1] Heidelberg Univ, Heidelberg Inst Plant Sci, Dept Cell Chem, D-69120 Heidelberg, Germany
关键词
glycosylation; mannosylation; mannosyltransferase; POMT2; POMT1;
D O I
10.1093/glycob/cwn042
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
O-Mannosylation represents an evolutionarily conserved, essential protein modi. cation. In mammals the protein O-mannosyltransferases POMT1 and POMT2 act as a heteromeric complex to initiate O-mannosylation in the endoplasmic reticulum. Mutations in human POMT1 and POMT2 cause a group of congenital muscular dystrophies due to reduced O-glycosylation of alpha-dystroglycan. The most severe of these autosomal recessive conditions is Walker-Warburg syndrome (WWS) with severe brain and ocular involvement. We previously showed in the murine model that Pomt1 is expressed in WWS-related tissues both during embryogenesis and in adults. Whereas there is only a single Pomt1 transcript in adult mice, we demonstrated that there are two Pomt2 transcripts, somatic sPomt2 and testis-specific tPomt2. In this study we demonstrate that sPomt2, but not tPomt2, is prominently expressed in mouse embryos in the tissues that are most severely affected in WWS(developing muscle, eye, and brain). Correlation of POMT transcripts and protein isoforms with POMT mannosyltransferase enzyme activity demonstrates that sPOMT2-POMT1 complexes catalyze mannosyltransfer in adult somatic tissues and testis. It is suggested that the gonadal defects described in some WWS cases are associated with defects in O-mannosylation. Our data further show that whereas sPOMT2 is widely expressed, tPOMT2 is restricted to the acrosome of male germ cells and is not involved in the biosynthesis of O-mannosyl glycans in vivo. We prove that tPOMT2 is highly conserved among mammals, including humans, suggesting a crucial function that is distinct from sPOMT2.
引用
收藏
页码:615 / 625
页数:11
相关论文
共 45 条
[1]   Mammalian sperm acrosome: Formation, contents, and function [J].
Abou-Haila, A ;
Tulsiani, DRP .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2000, 379 (02) :173-182
[2]   Physical and functional association of human protein O-mannosyltransferases 1 and 2 [J].
Akasaka-Manya, Keiko ;
Manya, Hiroshi ;
Nakajima, Ai ;
Kawakita, Masao ;
Endo, Tamao .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (28) :19339-19345
[3]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[4]   Dystroglycan protein distribution coincides with basement membranes and muscle differentiation during mouse embryogenesis [J].
Anderson, Claire ;
Winder, Steven J. ;
Borycki, Anne-Gaelle .
DEVELOPMENTAL DYNAMICS, 2007, 236 (09) :2627-2635
[5]   An autosomal recessive limb girdle muscular dystrophy (LGMD2) with mild mental retardation is allelic to Walker-Warburg syndrome (WWS) caused by a mutation in the POMT1 gene [J].
Balci, B ;
Uyanik, G ;
Dincer, P ;
Gross, C ;
Willer, T ;
Talim, B ;
Haliloglu, G ;
Kale, G ;
Hehr, U ;
Winkler, J ;
Topaloglu, H .
NEUROMUSCULAR DISORDERS, 2005, 15 (04) :271-275
[6]   Dystroglycan: from biosynthesis to pathogenesis of human disease [J].
Barresi, R ;
Campbell, KP .
JOURNAL OF CELL SCIENCE, 2006, 119 (02) :199-207
[7]   Dystroglycan expression in hepatic stellate cells:: Role in liver fibrosis [J].
Bedossa, P ;
Ferlicot, S ;
Paradis, V ;
Dargère, D ;
Bonvoust, F ;
Vidaud, M .
LABORATORY INVESTIGATION, 2002, 82 (08) :1053-1061
[8]   DISSOCIATION OF MOUSE TESTIS AND CHARACTERIZATION OF ISOLATED SPERMATOGENIC CELLS [J].
BELLVE, AR ;
MILLETTE, CF ;
BHATNAGAR, YM ;
OBRIEN, DA .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1977, 25 (07) :480-494
[9]   Mutations in the O-mannosyltransferase gene POMT1 give rise to the severe neuronal migration disorder Walker-Warburg syndrome [J].
Beltran-Valero de Bernabé, D ;
Currier, S ;
Steinbrecher, A ;
Celli, J ;
van Beusekom, E ;
van der Zwaag, B ;
Kayserili, H ;
Merlini, L ;
Chitayat, D ;
Dobyns, WB ;
Cormand, B ;
Lehesjoki, AE ;
Cruces, J ;
Voit, T ;
Walsh, CA ;
van Bokhoven, H ;
Brunner, HG .
AMERICAN JOURNAL OF HUMAN GENETICS, 2002, 71 (05) :1033-1043
[10]   Ectopic localizations of Golgi glycosyltransferases [J].
Berger, EG .
GLYCOBIOLOGY, 2002, 12 (02) :29R-36R