Development of specific oligonucleotide probes for the identification and in situ detection of hydrocarbon-degrading Alcanivorax strains

被引：76

作者：

Syutsubo, K ^{[1
]}

Kishira, H ^{[1
]}

Harayama, S ^{[1
]}

机构：

[1] Kamaishi Labs, Marine Biotechnol Inst, Kamaishi, Iwate 0260001, Japan

来源：

ENVIRONMENTAL MICROBIOLOGY | 2001年 / 3卷 / 06期

关键词：

D O I：

10.1046/j.1462-2920.2001.00204.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genus Alcanivorax comprises diverse hydrocarbon-degrading marine bacteria. Novel 16S rRNA-targeted oligonucleotide DNA probes (ALV735 and ALV735-b) were developed to quantify two subgroups of the Alcanivorax/Fundibacter group by fluorescence in situ hybridization (FISH), and the conditions for the single-mismatch discrimination of the probes were optimized. The specificity of the probes was improved further using a singly mismatched oligonucleotide as a competitor. The growth of Alcanivorax cells in crude oil-contaminated sea water under the biostimulation condition was investigated by FISH with the probe ALV735, which targeted the main cluster of the Alcanivorax/Fundibacter group. The size of the Alcanivorax population increased with increasing incubation time and accounted for 91% of the 4',6-diamidino-2-phenylindole (DAPI) count after incubation for 2 weeks. The probes developed in this study are useful for detecting Alcanivorax populations in petroleum hydrocarbon-degrading microbial consortia.

引用

页码：371 / 379

页数：9

共 24 条

[1] Novel glycine containing glucolipids from the alkane using bacterium Alcanivorax borkumensis [J].

Abraham, WR ;

Meyer, H ;

Yakimov, M .

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1998, 1393 (01) :57-62

[2]

Amann R. I., MOL MICROBIAL ECOLOG, P1

[3] FLUORESCENT-OLIGONUCLEOTIDE PROBING OF WHOLE CELLS FOR DETERMINATIVE, PHYLOGENETIC, AND ENVIRONMENTAL-STUDIES IN MICROBIOLOGY [J].

AMANN, RI ;

KRUMHOLZ, L ;

STAHL, DA .

JOURNAL OF BACTERIOLOGY, 1990, 172 (02) :762-770

[4] MOLECULAR AND MICROSCOPIC IDENTIFICATION OF SULFATE-REDUCING BACTERIA IN MULTISPECIES BIOFILMS [J].

AMANN, RI ;

STROMLEY, J ;

DEVEREUX, R ;

KEY, R ;

STAHL, DA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (02) :614-623

[5] PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].

AMANN, RI ;

LUDWIG, W ;

SCHLEIFER, KH .

MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169

[6] COMBINATION OF 16S RIBOSOMAL-RNA-TARGETED OLIGONUCLEOTIDE PROBES WITH FLOW-CYTOMETRY FOR ANALYZING MIXED MICROBIAL-POPULATIONS [J].

AMANN, RI ;

BINDER, BJ ;

OLSON, RJ ;

CHISHOLM, SW ;

DEVEREUX, R ;

STAHL, DA .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (06) :1919-1925

[7]

[Anonymous], 1990, PCR Protocols: a Guide to Methods and Application

[8] Fundibacter jadensis gen. nov., sp. nov., a new slightly halophilic bacterium, isolated from intertidal sediment [J].

Bruns, A ;

Berthe-Corti, L .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 :441-448

[9] Phylogenetic analysis of aerobic freshwater and marine enrichment cultures efficient in hydrocarbon degradation:: effect of profiling method [J].

Chang, YJ ;

Stephen, JR ;

Richter, AP ;

Venosa, AD ;

Brüggemann, J ;

Macnaughton, SJ ;

Kowalchuk, GA ;

Haines, JR ;

Kline, E ;

White, DC .

JOURNAL OF MICROBIOLOGICAL METHODS, 2000, 40 (01) :19-31

[10] PHYLOGENETIC STAINS - RIBOSOMAL RNA-BASED PROBES FOR THE IDENTIFICATION OF SINGLE CELLS [J].

DELONG, EF ;

WICKHAM, GS ;

PACE, NR .

SCIENCE, 1989, 243 (4896) :1360-1363

← 1 2 3 →