Phylogenetic diversity of bacterial communities in bovine rumen as affected by diets and microenvironments

被引：33

作者：

Kim, Minseok ^{[1
]}

Morrison, Mark ^{[1
,2
]}

Yu, Zhongtang ^{[1
]}

机构：

[1] Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA

[2] CSIRO Livestock Ind, St Lucia, Qld, Australia

来源：

FOLIA MICROBIOLOGICA | 2011年 / 56卷 / 05期

关键词：

MICROBIAL COMMUNITIES; ADHERENT; DNA;

D O I：

10.1007/s12223-011-0066-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Phylogenetic analysis was conducted to examine ruminal bacteria in two ruminal fractions (adherent fraction vs. liquid fraction) collected from cattle fed with two different diets: forage alone vs. forage plus concentrate. One hundred forty-four 16S rRNA gene (rrs) sequences were obtained from clone libraries constructed from the four samples. These rrs sequences were assigned to 116 different operational taxonomic units (OTUs) defined at 0.03 phylogenetic distance. Most of these OTUs could not be assigned to any known genus. The phylum Firmicutes was represented by approximately 70% of all the sequences. By comparing to the OTUs already documented in the rumen, 52 new OTUs were identified. UniFrac, SONS, and denaturing gradient gel electrophoresis analyses revealed difference in diversity between the two fractions and between the two diets. This study showed that rrs sequences recovered from small clone libraries can still help identify novel species-level OTUs.

引用

页码：453 / 458

页数：6

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