A molecular imprinted SPR biosensor for sensitive determination of citrinin in red yeast rice

被引：80

作者：

Atar, Necip ^{[1
]}

Eren, Tanju ^{[1
]}

Yola, Mehmet Lutfi ^{[2
]}

机构：

[1] Pamukkale Univ, Fac Engn, Dept Chem Engn, Denizli, Turkey

[2] Sinop Univ, Fac Engn, Dept Met & Mat Engn, Sinop, Turkey

来源：

FOOD CHEMISTRY | 2015年 / 184卷

关键词：

Mycotoxin; Red yeast rice; Food analysis; Biosensor; SURFACE-PLASMON RESONANCE; CHROMATOGRAPHIC DETERMINATION; MYCOTOXIN CITRININ; NATURAL OCCURRENCE; NANOSENSOR; IMMUNOASSAY; OCHRATOXIN; EZETIMIBE; AFLATOXIN; PRODUCTS;

D O I：

10.1016/j.foodchem.2015.03.065

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A novel and sensitive molecular imprinted surface plasmon resonance (SPR) biosensor was developed for selective determination of citrinin (CIT) in red yeast rice. Firstly, the gold surface of SPR chip was modified with allyl mercaptane. Then, CIT-imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoglutamic acid) (p(HEMA-MAGA)) film was generated on the gold surface modified with allyl mercaptane. The unmodified and imprinted surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and contact angle measurements. The linearity range and the detection limit were obtained as 0.005-1.0 ng/mL and 0.0017 ng/mL, respectively. The SPR biosensor was applied to determination of CIT in red yeast rice sample. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：7 / 11

页数：5

共 29 条

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AN INDIRECT ENZYME-IMMUNOASSAY FOR THE MYCOTOXIN CITRININ [J].