20-hydroxyecdysone 2-beta-D-glycopyranoside from seeds of Xerophyllum tenax

被引：9

作者：

Alison, B

Whiting, P

Sarker, SD

Dinan, L

Underwood, E

Sik, V

Rees, HH

机构：

[1] UNIV EXETER, DEPT SCI BIOL, WASHINGTON SINGER LABS, EXETER EX4 4QG, DEVON, ENGLAND

[2] UNIV EXETER, DEPT CHEM, EXETER EX4 4QD, DEVON, ENGLAND

[3] UNIV LIVERPOOL, DEPT BIOCHEM, LIVERPOOL L69 3BX, MERSEYSIDE, ENGLAND

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 1997年 / 25卷 / 03期

关键词：

Xerophyllum tenax; Melanthiaceae; Liliaceae; ecdysteroid; steroid; seeds; Drosophila melanogaster; bioassay; chemotaxonomy;

D O I：

10.1016/S0305-1978(97)00003-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bioassay/RIA-directed HPLC purification of a MeOH extract of the seeds of Xerophyllum tenax yielded 20-hydroxyecdysone and 20-hydroxyecdysone 2-beta-D-glucopyranoside. the latter being a novel natural product. The structures were elucidated primarily on the basis of 1D and 2D NMR spectroscopic evidence. The ecdysteroid agonist activity of these isolates was determined on the B-11 tumorous blood cell line of Drosophila melanogaster. ED50 values for 20-hydroxyecdysone and 20-hydroxyecdysone 2-beta-D-glucopyranoside were found to be 8.0x10(-9) M and 1.3x10(-4) M, respectively. The significance of the presence of ecdysteroid conjugates in plants is discussed. (C) 1997 Elsevier Science Ltd.

引用

页码：255 / 261

页数：7

共 32 条

[1]

BAILEY LH, 1977, MANUAL CULTIVATED PL, P203

[2] H-1 AND C-13 ASSIGNMENTS FROM SENSITIVITY-ENHANCED DETECTION OF HETERONUCLEAR MULTIPLE-BOND CONNECTIVITY BY 2D MULTIPLE QUANTUM NMR [J].

BAX, A ;

SUMMERS, MF .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (08) :2093-2094

[3]

BERGAMASCO R, 1983, ENDOCRINOLOGY INSECT, V1, P627

[4]

BLACKFORD M, 1997, IN PRESS INSECT BIOC

[5]

Blackford MJP, 1997, ARCH INSECT BIOCHEM, V34, P329, DOI 10.1002/(SICI)1520-6327(1997)34:3<329::AID-ARCH7>3.0.CO

[6]

2-P

[7]

Brummitt R.K., 1992, VASCULAR PLANT FAMIL, P456

[8] ASSESSMENT OF A MICROPLATE-BASED BIOASSAY FOR THE DETECTION OF ECDYSTEROID-LIKE OR ANTIECDYSTEROID ACTIVITIES [J].

CLEMENT, CY ;

BRADBROOK, DA ;

LAFONT, R ;

DINAN, L .

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1993, 23 (01) :187-192

[9]

Cronquist A., 1981, INTEGRATED SYSTEM CL

[10]

DINAN L, 1995, EUR J ENTOMOL, V92, P295

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