Effects of bisphenol A and its derivatives on the response of GABAA receptors expressed in Xenopus oocytes

被引:26
作者
Aoshima, H
Hossain, SJ
Imamura, H
Shingai, R
机构
[1] Yamaguchi Univ, Fac Sci, Dept Phys Biol & Informat, Yamaguchi 7538512, Japan
[2] Iwate Univ, Fac Engn, Dept Welfare Engn, Morioka, Iwate 0208551, Japan
关键词
bisphenol-A; 3,5-dimethylphenol; GABA(A) receptor; p-t-butylphenol; Xenopus oocyte;
D O I
10.1271/bbb.65.2070
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To study the effects of bisphenol-A (BPA) known to have estrogenic actions, and its derivatives, 3,5-dimethylphenol (DMP) and p-t-butylphenol (TBP), on ionotropic gamma -aminobutyric acid (GABA) receptors, GABAA receptors were expressed in Xenopus oocytes by injecting both poly(A)(+)RNA prepared from rat whole brain and cRNAs synthesized from cloned cDNAs of alpha (1) and beta (1) subunit of the bovine receptors, and their electrical responses were measured by the voltage clamping method. BPA caused the potentiation and inhibition of the former receptor-responses, while it caused only inhibition of the latter ones. In the presence of low concentrations of GABA, DMP and TBP potentiated the responses of both receptors. DMP and TBP also increased the rate of decay of the response, possibly by desensitization of the receptors when GABA solution was continuously bath-applied. Diethyl terephthalate (DTP), which is also known to have estrogenic actions, had little effect on both the responses and the decay of both receptors.
引用
收藏
页码:2070 / 2077
页数:8
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