A simplified procedure for the isolation of immunoglobulins from human serum using a novel type of thiophilic gel at low salt concentration

被引:28
作者
Scholz, GH
Vieweg, S
Leistner, S
Seissler, J
Scherbaum, WA
Huse, K
机构
[1] Univ Leipzig, Dept Internal Med 3, D-04103 Leipzig, Germany
[2] Univ Leipzig, Dept Pharmaceut Chem, Inst Pharm, D-04103 Leipzig, Germany
[3] Univ Dusseldorf, Diabet Res Inst, D-4000 Dusseldorf, Germany
关键词
immunoglobulin; antibody; affinity chromatography; thiophilic adsorption; autoantibodies;
D O I
10.1016/S0022-1759(98)00132-X
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
By coupling 3-(2-mercaptoethyl)quinazoline-2,4(1H,3H)dione (MECH) to divinyl sulfone activated agarose, a novel thiophilic matrix was obtained which allows the binding of immunoglobulins from different sources. In contrast to other thiophilic gels, antibodies are bound at low ionic strength and can easily be desorbed in intact form by elution with dilute alkali. The potential of using the MECH-gel was demonstrated by the purification of antibodies from human and animal (goat, rabbit, mouse) sera. The functional integrity of the purified antibodies was established with cytoplasmic islet cell antibodies from the sera of patients with type I diabetes and autoantibodies against thyroid peroxidase from patients with Graves' and Hashimoto's disease. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:109 / 118
页数:10
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