Chemoprotective effects of hesperidin against genotoxicity induced by cyclophosphamide in mice bone marrow cells

被引:71
作者
Ahmadi, Amirhosein [2 ]
Hosseinimehr, Seyed Jalal [1 ,3 ]
Naghshvar, Farshad [4 ]
Hajir, Ebrahim [2 ]
Ghahremani, Mehran [4 ]
机构
[1] Mazandaran Univ Med Sci, Dept Med Chem, Fac Pharm, Sari, Iran
[2] Mazandaran Univ Med Sci, Fac Pharm, Student Res Comm, Sari, Iran
[3] Mazandaran Univ Med Sci, Pharmaceut Res Ctr, Sari, Iran
[4] Mazandaran Univ Med Sci, Dept Pathol, Fac Med, Sari, Iran
关键词
hesperidin; genotoxicity; micronucleus; cyclophosphamide;
D O I
10.1007/s12272-001-1228-z
中图分类号
R914 [药物化学];
学科分类号
100701 [药物化学];
摘要
The preventive effect of hesperidin as a flavonoid was investigated in mouse bone marrow cells against genotoxicty induced by cyclophosphamide. Mice were orally (gavages) pre-treated with solutions of hesperidin at four different doses (50, 100, 200, and 400 mg/kg b.w.) for five consecutive days. Mice were injected intraperitoneally on the fifth day with cyclophosphamide (50 mg/kg b.w.) and killed after 24 h for the evaluation of micronucleated polychromatic erythrocytes (MnPCEs) and the ratio of PCE/(PCE+NCE) (polychromatic erythrocyte/ polychromatic erythrocyte + normochromatic erythrocyte). Three last doses of hesperidin significantly reduced frequency of MnPCEs induced by cyclophosphamide (p < 0.0001). Hesperdin at dose 200 mg/kg b.w. reduced MnPCEs 2.37 time and also completely normalized PCE/ (PCE+NCE) ratio. Histological examination of bone marrow showed that hesperidin affected on proliferation and hyper cellularity of immature myeloid elements in bone marrow that reduced by cyclophsopahmide. It is obvious that hesperidin, may with antioxidative activity, reduced the oxidative stress and genotoxicity induced by cyclophosphamide in mouse bone marrow cells.
引用
收藏
页码:794 / 797
页数:4
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