Thyroid hormone promotes cell invasion through activation of furin expression in human hepatoma cell lines

被引：54

作者：

Chen, Ruey-Nan ^{[1
]}

Huang, Ya-Hui ^{[1
]}

Lin, Ya-Chu ^{[1
]}

Yeh, Chau-Ting ^{[2
,5
]}

Liang, Ying ^{[3
]}

Chen, Shen-Liang ^{[4
]}

Lin, Kwang-Huei ^{[1
]}

机构：

[1] Chang Gung Univ, Dept Biochem, Tao Yuan 333, Taiwan

[2] Chang Gung Univ, Dept Med, Tao Yuan 333, Taiwan

[3] Chang Gung Univ, Sch Med, Proteom Core Lab, Tao Yuan 333, Taiwan

[4] Natl Cent Univ, Dept Life Sci, Jhongli 320, Taiwan

[5] Chang Gung Med Ctr, Liver Res Unit, Taipei 10591, Taiwan

来源：

ENDOCRINOLOGY | 2008年 / 149卷 / 08期

关键词：

D O I：

10.1210/en.2007-0989

中图分类号：

R5 [内科学];

学科分类号：

1002 [临床医学]; 100201 [内科学];

摘要：

The objective of this study was to identify genes regulated by thyroid hormone (T-3) and associated with tumor invasion. The gene encoding furin, as previously identified by cDNA microarray, is known to be up-regulated by T-3 treatment, and stimulated furin production occurs in thyroidectomized rats after administration of T-3. Presently, by using serial deletion of the promoter and EMSAs, the T-3 response element on the furin promoter was localized to the -6317/-6302 region. T-3-mediated furin up-regulation was cooperative with TGF-beta because T-3 induction increased after Smad3/4 addition. Furthermore, the invasiveness of HepG2-thyroid hormone receptor (TR) cells was significantly increased by T-3 treatment, perhaps due to furin processing of matrix metalloproteinase-2 and -9. In addition, furin up-regulation either by stable overexpression

引用

页码：3817 / 3831

页数：15

共 54 条

[1]

Activation of the furin endoprotease is a multiple-step process: Requirements for acidification and internal propeptide cleavage [J].