Atypical development of the tracheal basement membrane zone of infant rhesus monkeys exposed to ozone and allergen

被引:32
作者
Evans, MJ [1 ]
Fanucchi, MV
Baker, GL
Van Winkle, LS
Pantle, LM
Nishio, SJ
Schelegle, ES
Gershwin, LJ
Miller, LA
Hyde, DM
Sannes, PL
Plopper, CG
机构
[1] Univ Calif Davis, Sch Vet Med, Dept Anat Physiol & Cell Biol, Ctr Comparat Resp Biol & Med, Davis, CA 95616 USA
[2] N Carolina State Univ, Coll Vet Med, Dept Mol Biomed Sci, Raleigh, NC 27606 USA
关键词
perlecan; fibroblast growth factor-2; fibroblast growth factor; receptor-1; syndecan-4; epithelial-mesenchymal trophic unit;
D O I
10.1152/ajplung.00175.2003
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Development of the basement membrane zone (BMZ) occurs postnatally in the rhesus monkey. The purpose of this study was to determine whether house dust mite allergen (HDMA) plus ozone altered this process. Rhesus monkeys were exposed to a regimen of HDMA and/or ozone or filtered air for 6 mo. To detect structural changes in the BMZ, we measured immunoreactivity of collagen I. To detect functional changes in the BMZ, we measured perlecan and fibroblast growth factor-2 (FGF-2). We also measured components of the FGF-2 ternary signaling complex [fibroblast growth factor receptor-1 (FGFR-1) and syndecan-4]. The width of the BMZ was irregular in the ozone groups, suggesting atypical development of the BMZ. Perlecan was also absent from the BMZ. In the absence of perlecan, FGF-2 was not bound to the BMZ. However, FGF-2 immunoreactivity was present in basal cells, the lateral intercellular space (LIS), and attenuated fibroblasts. FGFR-1 immunoreactivity was downregulated, and syndecan-4 immunoreactivity was upregulated in the basal cells. This suggests that FGF-2 in basal cells and LIS may be bound to the syndecan-4. We conclude that ozone and HDMA plus ozone effected incorporation of perlecan into the BMZ, resulting in atypical development of the BMZ. These changes are associated with specific alterations in the regulation of FGF-2, FGFR-1, and syndecan-4 in the airway epithelial-mesenchymal trophic unit, which may be associated with the developmental problems of lungs associated with exposure to ozone.
引用
收藏
页码:L931 / L939
页数:9
相关论文
共 52 条
[1]  
Adachi E, 1997, INT REV CYTOL, V173, P73
[2]   Cytoplasmic interactions of syndecan-4 orchestrate adhesion receptor and growth factor receptor signalling [J].
Bass, MD ;
Humphries, MJ .
BIOCHEMICAL JOURNAL, 2002, 368 :1-15
[3]   Biological roles of fibroblast growth factor-2 [J].
Bikfalvi, A ;
Klein, S ;
Pintucci, G ;
Rifkin, DB .
ENDOCRINE REVIEWS, 1997, 18 (01) :26-45
[4]   Asthma - From bronchoconstriction to airways inflammation and remodeling [J].
Bousquet, J ;
Jeffery, PK ;
Busse, WW ;
Johnson, M ;
Vignola, AM .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2000, 161 (05) :1720-1745
[5]   MYOFIBROBLASTS AND SUBEPITHELIAL FIBROSIS IN BRONCHIAL-ASTHMA [J].
BREWSTER, CEP ;
HOWARTH, PH ;
DJUKANOVIC, R ;
WILSON, J ;
HOLGATE, ST ;
ROCHE, WR .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1990, 3 (05) :507-511
[6]   Perlecan maintains the integrity of cartilage and some basement membranes [J].
Costell, M ;
Gustafsson, E ;
Aszódi, A ;
Mörgelin, M ;
Bloch, W ;
Hunziker, E ;
Addicks, K ;
Timpl, R ;
Fässler, R .
JOURNAL OF CELL BIOLOGY, 1999, 147 (05) :1109-1122
[7]  
Couchman JR, 2001, INT REV CYTOL, V207, P113
[8]  
CROUCH EC, 1997, LUNG, P769
[9]   Heparan sulfate mediates bFGF transport through basement membrane by diffusion with rapid reversible binding [J].
Dowd, CJ ;
Cooney, CL ;
Nugent, MA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (08) :5236-5244
[10]   The attenuated fibroblast sheath of the respiratory tract epithelial-mesenchymal trophic unit [J].
Evans, MJ ;
Van Winkle, LS ;
Fanucchi, MV ;
Plopper, CG .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1999, 21 (06) :655-657