Constitutive and inducible green fluorescent protein expression in Bartonella henselae

被引：36

作者：

Lee, AK ^{[1
]}

Falkow, S ^{[1
]}

机构：

[1] Stanford Univ, Dept Microbiol & Immunol, Stanford, CA 94305 USA

来源：

INFECTION AND IMMUNITY | 1998年 / 66卷 / 08期

关键词：

D O I：

10.1128/IAI.66.8.3964-3967.1998

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The green fluorescent protein (GFP) gene was expressed on a plasmid in B. henselae, and GFP-expressing bacteria were visualized by fluorescence microscopy. HEp-2 cells infected with GFP-expressing bacteria were separated from uninfected cells with a fluorescence activated cell sorter. Promoter fusions of B. henselae chromosomal DNA to gfp were examined by Bow cytometry, and a B. henselae groEL promoter fusion which induced expression at 37 degrees C was isolated.

引用

页码：3964 / 3967

页数：4

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