Endocytosis: clathrin-mediated membrane budding

被引:187
作者
Ungewickell, Ernst J.
Hinrichsen, Lars
机构
[1] Hannover Med Sch, Dept Cell Biol, Ctr Anat, D-30625 Hannover, Germany
[2] UCL, MRC, Mol Cell Biol Lab, London WC1E 6BT, England
关键词
D O I
10.1016/j.ceb.2007.05.003
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Clathrin-dependent endocytosis is the major pathway for the uptake of nutrients and signaling molecules in higher eukaryotic cells. The long-held tenet that clathrin-coated vesicles are created from flat coated plasma membrane patches by a sequential process of invagination, bud formation and fission recently received strong support from the results of advanced live cell fluorescence microscopy. The data on the critical components that deform the plasma membrane locally into a coated bud suggest that membrane bending is a team effort requiring membrane-curving protein domains, actin dynamics and, last but not least, clathrin. The scission step requires the mechano-enzymatic function of dynamin, actin dynamics and possibly myosin motor proteins. Finally, a burst of auxilin/GAK initiates the uncoating of the vesicle.
引用
收藏
页码:417 / 425
页数:9
相关论文
共 83 条
[1]   Type Iγ661 phosphatidylinositol phosphate kinase directly interacts with AP2 and regulates endocytosis [J].
Bairstow, Shawn F. ;
Ling, Kun ;
Su, Xiaojing ;
Firestone, Ari J. ;
Carbonara, Chateen ;
Anderson, Richard A. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (29) :20632-20642
[2]   Plasma membrane domains specialized for clathrinmediated endocytosis in primary cells [J].
Bellve, Karl D. ;
Leonard, Deborah ;
Standley, Clive ;
Lifshitz, Lawrence M. ;
Tuft, Richard A. ;
Hayakawa, Akira ;
Corvera, Silvia ;
Fogarty, Kevin E. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (23) :16139-16146
[3]   N-WASP deficiency impairs EGF internalization and actin assembly at clathrin-coated pits [J].
Benesch, S ;
Polo, S ;
Lai, FPL ;
Anderson, KI ;
Stradal, TEB ;
Wehland, J ;
Rottner, K .
JOURNAL OF CELL SCIENCE, 2005, 118 (14) :3103-3115
[4]   The ear of alpha-adaptin interacts with the COOH-terminal domain of the Eps15 protein [J].
Benmerah, A ;
Begue, B ;
DautryVarsat, A ;
CerfBensussan, N .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (20) :12111-12116
[5]  
BRETSCHER MS, 1981, COLD SPRING HARB SYM, V46, P707
[6]   Structural definition of the F-actin-binding THATCH domain from HIP1R [J].
Brett, TJ ;
Legendre-Guillemin, V ;
McPherson, PS ;
Fremont, DH .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2006, 13 (02) :121-130
[7]   Accessory protein recruitment motifs in clathrin-mediated endocytosis [J].
Brett, TJ ;
Traub, LM ;
Fremont, DH .
STRUCTURE, 2002, 10 (06) :797-809
[8]   Coincidence detection in phosphoinositide signaling [J].
Carlton, JG ;
Cullen, PJ .
TRENDS IN CELL BIOLOGY, 2005, 15 (10) :540-547
[9]   Downregulation of CD4 by human immunodeficiency virus type 1 Nef is dependent on clathrin and involves direct interaction of Nef with the AP2 clathrin adaptor [J].
Chaudhuri, Rittik ;
Lindwasser, O. Wolf ;
Smith, William J. ;
Hurley, James H. ;
Bonifacino, Juan S. .
JOURNAL OF VIROLOGY, 2007, 81 (08) :3877-3890
[10]   Epsin is an EH-domain-binding protein implicated in clathrin-mediated endocytosis [J].
Chen, H ;
Fre, S ;
Slepnev, VI ;
Capua, MR ;
Takei, K ;
Butler, MH ;
Di Fiore, PP ;
De Camilli, P .
NATURE, 1998, 394 (6695) :793-797