Quantitation by image analysis of global DNA methylation in human spermatozoa and its prognostic value in in vitro fertilization:: a preliminary study

被引：68

作者：

Benchaib, M ^{[1
]}

Ajina, M ^{[1
]}

Lornage, J ^{[1
]}

Niveleau, A ^{[1
]}

Durand, P ^{[1
]}

Guérin, JF ^{[1
]}

机构：

[1] Reprod Biol Lab, F-69373 Lyon 08, France

来源：

FERTILITY AND STERILITY | 2003年 / 80卷 / 04期

关键词：

human sperm; DNA methylation quantitation; image analysis; CASA; fertilization;

D O I：

10.1016/S0015-0282(03)01151-8

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To determine the relationship between sperm DNA methylation level and sperm characteristics and pregnancy rates. Design: Prospective study. Quantitation by image analysis of DNA methylation in sperm nucleus. Setting: Department of Reproduction Biology, Edouard Herriot Hospital, Lyon, France. Patient(s): Infertile couples undergoing IVF-ET. Intervention(s): The immunostaining of 5 methyl-cytosine was performed on the spare sperm suspension that was used for an assisted reproduction technology procedure. Main Outcome Measure(s): Sperm characteristics according to World Health Organization criteria, sperm motility parameters with computer-assisted semen analysis, sperm DNA methylation level, and heterogeneity index (HI). Result(s): Sperm DNA methylation level and HI are correlated with sperm DNA characteristics. HI is negatively correlated with fertilization rate; sperm DNA methylation level is correlated with pregnancy rate. Conclusion(s): The DNA methylation level in human spermatozoa could be a new approach to evaluating the ability of spermatozoa to fertilize and lead to normal embryo development. (C)2003 by American Society for Reproductive Medicine.

引用

页码：947 / 953

页数：7

共 15 条

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