Cytokinins act directly on lateral root founder cells to inhibit root initiation

被引：436

作者：

Laplaze, Laurent ^{[1
]}

Benkova, Eva ^{[2
,3
]}

Casimiro, Ilda ^{[4
]}

Maes, Lies ^{[2
,3
]}

Vanneste, Steffen ^{[2
,3
]}

Swarup, Ranjan ^{[5
]}

Weijers, Dolf ^{[6
]}

Calvo, Vanessa ^{[4
]}

Parizot, Boris ^{[2
,3
,7
]}

Herrera-Rodriguez, Maria Begona ^{[1
]}

Offringa, Remko ^{[6
]}

Graham, Neil ^{[8
]}

Doumas, Patrick ^{[1
]}

Friml, Jiri ^{[2
,3
]}

Bogusz, Didier ^{[1
]}

Beeckman, Tom ^{[2
,3
]}

Bennett, Malcolm ^{[5
]}

机构：

[1] Univ Montpellier 2, Inst Natl Rech Agron,Inst Rech Dev, Unite Mixte Rech Divers & Adaptat Plantes Cultive, Equipe Rhizogenese, F-34394 Montpellier 5, France

[2] Vlaams Inst Biotechnol, Root Dev Grp, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[3] Univ Ghent, Dept Mol Genet, B-9052 Ghent, Belgium

[4] Univ Extremadura, Dept Ciencias Morfolog & Biol Celular & Anim, E-06071 Badajoz, Spain

[5] Univ Nottingham, Sch Biosci, Ctr Plant Integrat Biol, Loughborough LE12 5RD, Leics, England

[6] Leiden Univ, Inst Biol, NL-2333 AL Leiden, Netherlands

[7] Commissariat Energie Atom Cadarache, CNRS, Commissariat Energie Atom Aix Marseille 2, Lab Biol Dev Plantes, F-13108 St Paul Les Durance, France

[8] Univ Nottingham, Sch Biosci, Nottingham Arabidopsis Stock Ctr, Loughborough LE12 5RD, Leics, England

来源：

PLANT CELL | 2007年 / 19卷 / 12期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1105/tpc.107.055863

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In Arabidopsis thaliana, lateral roots are formed from root pericycle cells adjacent to the xylem poles. Lateral root development is regulated antagonistically by the plant hormones auxin and cytokinin. While a great deal is known about how auxin promotes lateral root development, the mechanism of cytokinin repression is still unclear. Elevating cytokinin levels was observed to disrupt lateral root initiation and the regular pattern of divisions that characterizes lateral root development in Arabidopsis. To identify the stage of lateral root development that is sensitive to cytokinins, we targeted the expression of the Agrobacterium tumefaciens cytokinin biosynthesis enzyme isopentenyltransferase to either xylem-pole pericycle cells or young lateral root primordia using GAL4-GFP enhancer trap lines. Transactivation experiments revealed that xylem-pole pericycle cells are sensitive to cytokinins, whereas young lateral root primordia are not. This effect is physiologically significant because transactivation of the Arabidopsis cytokinin degrading enzyme cytokinin oxidase 1 in lateral root founder cells results in increased lateral root formation. We observed that cytokinins perturb the expression of PIN genes in lateral root founder cells and prevent the formation of an auxin gradient that is required to pattern lateral root primordia.

引用

页码：3889 / 3900

页数：12

共 48 条

[1] T-DNA OF AGROBACTERIUM-TUMEFACIENS ENCODES AN ENZYME OF CYTOKININ BIOSYNTHESIS [J].