Genotoxicity study with special reference to DNA damage by comet assay in fission yeast, Schizosaccharomyces pombe exposed to drinking water

被引：13

作者：

Banerjee, Pamela ^{[2
]}

Talapatra, Sournendra N.

Mandal, Nivedita ^{[1
,2
]}

Sundaram, Geetanjali ^{[3
]}

Mukhopadhyay, Aniruddha ^{[2
]}

Chattopadhyay, Dhrubaj'yoti ^{[3
]}

Banerjee, Sudip K. ^{[4
]}

机构：

[1] Ghosh Bose & Assoc Pvt Ltd, Environm Div, Kolkata 700071, W Bengal, India

[2] Univ Calcutta, Dept Environm Sci, Kolkata 700019, W Bengal, India

[3] Univ Calcutta, Dr BC Guha Ctr Genet Engn & Biotechnol, Kolkata 700019, W Bengal, India

[4] W Bengal Pollut Control Board, Kolkata 700098, W Bengal, India

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2008年 / 46卷 / 01期

关键词：

genotoxicity; drinking water; DNA damage; comet assay; fission yeast; Schizosaccharomyces pombe;

D O I：

10.1016/j.fct.2007.08.026

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The objective of this study was to investigate genotoxicity, especially DNA damage, in drinking water samples collected from tap by using fission yeast Schizosaccharomyces pombe as a model organism. Generally raw water potabolization is done by treatment with polymeric coagulant, alum, chlorine, etc. In the comet test, highly significant (P < 0.001) effects of DNA damage were detected in treated water (tap water) when compared to negative control (raw water) as well as laboratory control (distilled water) samples for both I It and 2 h exposure. In the water treatment plant, raw water treatment is done by the process of prechlorination, alum and polymeric coagulant (CatflocT) dosing, postchlorination, filtration and final discharge for consumption. In conclusion it can be stated from the results that chlorinated disinfectant, alum and polymeric coagulant (CatflocT) mixture used in drinking water has a potent cumulative genotoxic effect in the eukaryotic cells and may pose potential genotoxic risk for human health following long-term consumption. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：402 / 407

页数：6

共 32 条

[1] Comet assay responses as indicators of carcinogen exposure [J].

Anderson, D ;

Yu, TW ;

McGregor, DB .

MUTAGENESIS, 1998, 13 (06) :539-555

[2]

[Anonymous], GUID DRINK WAT QUAL

[3] Comet and micronucleus assays in zebra mussel cells for genotoxicity assessment of surface drinking water treated with three different disinfectants [J].

Bolognesi, C ;

Buschini, A ;

Branchi, E ;

Carboni, P ;

Furlini, M ;

Martino, A ;

Monteverde, M ;

Poli, P ;

Rossi, C .

SCIENCE OF THE TOTAL ENVIRONMENT, 2004, 333 (1-3) :127-136

[4] Genotoxicity and cytotoxicity assessment in lake drinking water produced in a treatment plant [J].

Buschini, A ;

Carboni, P ;

Frigerio, S ;

Furlini, M ;

Marabini, L ;

Monarca, S ;

Poli, P ;

Radice, S ;

Rossi, C .

MUTAGENESIS, 2004, 19 (05) :341-347

[5] The epidemiology of chemical contaminants of drinking water [J].

Calderon, RL .

FOOD AND CHEMICAL TOXICOLOGY, 2000, 38 :S13-S20

[6] Drinking water and cancer [J].

Cantor, KP .

CANCER CAUSES & CONTROL, 1997, 8 (03) :292-308

[7] Biological drinking water treatment processes, with special reference to mutagenicity [J].

Carraro, E ;

Bugliosi, EH ;

Meucci, L ;

Baiocchi, C ;

Gilli, G .

WATER RESEARCH, 2000, 34 (11) :3042-3054

[8] Meiotic recombination and chromosome segregation in Schizosaccharomyces pombe [J].

Davis, L ;

Smith, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (15) :8395-8402

[9]

FANTES P, 2000, YEAST NUCL

[10] In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis. [J].

Fischer, D ;

Li, YX ;

Ahlemeyer, B ;

Krieglstein, J ;

Kissel, T .

BIOMATERIALS, 2003, 24 (07) :1121-1131

← 1 2 3 4 →