A multicolor fluorescence immunostaining technique for simultaneous antigen targeting

被引：30

作者：

Buchwalow, IB ^{[1
]}

Minin, EA ^{[1
]}

Boecker, W ^{[1
]}

机构：

[1] Univ Munster, Gerhard Domagk Inst Pathol, Interdisciplinary Ctr Clin Res, D-48149 Munster, Germany

来源：

ACTA HISTOCHEMICA | 2005年 / 107卷 / 02期

关键词：

immunocytochemistry; immunohistology; multiple immuno-labeling; monoclonal primary antibodies;

D O I：

10.1016/j.acthis.2005.01.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

immunolabeling method with primary antibodies originating from the same host species. Here, we briefly outline different approaches intended to close this technological gap and focus on multiple immunolabeling with monoclonal primary antibodies. To this end, we generated a basic universal protocol for the use of secondary antibodies selectively recognizing different isotypes/subclasses of monoclonal primary antibodies. This approach is widely applicable and offers a simple procedure for simultaneously detecting two or more antigens. (c) 2005 Elsevier GmbH. All rights reserved.

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收藏

页码：143 / 148

页数：6

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