A role for the cleaved cytoplasmic domain of E-cadherin in the nucleus

被引：117

作者：

Ferber, Emma C. ^{[1
]}

Kajita, Mihoko ^{[1
]}

Wadlow, Anthony ^{[1
]}

Tobiansky, Lara ^{[1
]}

Niessen, Carien ^{[3
]}

Ariga, Hiroyoshi ^{[4
]}

Daniel, Juliet ^{[5
]}

Fujita, Yasuyuki ^{[1
,2
]}

机构：

[1] UCL, MRC Cell Biol Unit, MRC Lab Mol Cell Biol, London WC1E 6BT, England

[2] UCL, Dept Cell & Dev Biol, London WC1E 6BT, England

[3] Univ Cologne, Dept Dermatol, Ctr Mol Med Cologne, D-50931 Cologne, Germany

[4] Hokkaido Univ, Grad Sch Pharmaceut Sci, Kita Ku, Sapporo, Hokkaido 0600812, Japan

[5] McMaster Univ, Dept Biol, Hamilton, ON L8S 4K1, Canada

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2008年 / 283卷 / 19期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1074/jbc.M708887200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cell-cell contacts play a vital role in intracellular signaling, although the molecular mechanisms of these signaling pathways are not fully understood. E-cadherin, an important mediator of cell-cell adhesions, has been shown to be cleaved by gamma-secretase. This cleavage releases a fragment of E-cadherin, E-cadherin C-terminal fragment 2 (E-cad/CTF2), into the cytosol. Here, we study the fate and function of this fragment. First, we show that coexpression of the cadherin-binding protein, p120 catenin (p120), enhances the nuclear translocation of E-cad/CTF2. By knocking down p120 with short interfering RNA, we also demonstrate that p120 is necessary for the nuclear localization of E-cad/CTF2. Furthermore, p120 enhances and is required for the specific binding of E-cad/CTF2 to DNA. Finally, we show that E-cad/CTF2 can regulate the p120-Kaiso-mediated signaling pathway in the nucleus. These data indicate a novel role for cleaved E-cadherin in the nucleus.

引用

页码：12691 / 12700

页数：10

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