Induction of CD4+ T cell-dependent CD8+ type 1 responses in humans by a malaria DNA vaccine

被引：172

作者：

Wang, RB

Epstein, J

Baraceros, FM

Gorak, EJ

Charoenvit, Y

Carucci, DJ

Hedstrom, RC

Rahardjo, N

Gay, T

Hobart, P

Stout, R

Jones, TR

Richie, TL

Parker, SE

Doolan, DL

Norman, J

Hoffman, SL

机构：

[1] Johns Hopkins Univ, Sch Hyg & Publ Hlth, Baltimore, MD 21205 USA

[2] Bioject Inc, Portland, OR 97224 USA

[3] Vical Inc, San Diego, CA 92121 USA

[4] USA, Med Res Inst Infect Dis, Ft Detrick, MD 21702 USA

[5] Henry M Jackson Fdn, Rockville, MD 20852 USA

[6] USN, Med Res Ctr, Malaria Program, Silver Spring, MD 20910 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 19期

关键词：

D O I：

10.1073/pnas.181123498

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We assessed immunogenicity of a malaria DNA vaccine administered by needle i.m. or needleless jet injection [i.m. or i.m./intradermally (i.d.)] in 14 volunteers. Antigen-specific IFN-gamma responses were detected by enzyme-linked immunospot (ELISPOT) assays in all subjects to multiple 9- to 23-aa peptides containing class I and/or class II restricted epitopes, and were dependent on both CD8(+) and CD4(+) T cells. Overall, frequency of response was significantly greater after i.m. jet injection. CD8(+)-dependent cytotoxic T lymphocytes (CTL) were detected in 8/14 volunteers. Demonstration in humans of elicitation of the class I restricted IFN-gamma responses we believe necessary for protection against the liver stage of malaria parasites brings us closer to an effective malaria vaccine.

引用

页码：10817 / 10822

页数：6

共 30 条

[1] Vaccination of seronegative volunteers with a human immunodeficiency virus type 1 env/rev DNA vaccine induces antigen-specific proliferation and lymphocyte production of β-chemokines [J].