Identification of low-dye-binding (ldb) mutants of Saccharomyces cerevisiae

被引：18

作者：

Corbacho, I ^{[1
]}

Olivero, I ^{[1
]}

Hernández, LM ^{[1
]}

机构：

[1] Univ Extremadura, Dept Microbiol, E-06071 Badajoz, Spain

来源：

FEMS YEAST RESEARCH | 2004年 / 4卷 / 4-5期

关键词：

Saccharomyces cerevisiae; glycosylation; deletion mutant; ldb mutant; oligosaccharide phosphorylation;

D O I：

10.1016/S1567-1356(03)00162-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have completed the identification of Saccharomyces cerevisiae genes that are defective in previously isolated ldb (low-dye-binding) mutants. This was done by complementation of the mutant's phenotype with DNA fragments from a genomic library and by running standard tests of allelism with single-gene deletion mutants of similar phenotype. The results were as follows: LDB2 is allelic to ERD1; LDB4 to SPC72; LDB5 to RLR1; LDB6 to GON7/YJL184W; LDB7 to YBL006C; LDB9 to ELM1; LDB10 to CWH36; LDB11 to COG1; LDB12 to OCH1; LDB13 to VAN1; LDB14 to BUD32; and LDB15 to PHO85. Since the precise function of some of the genes is not known, these data may contribute to the functional characterization of the S. cerevisiae genome. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：437 / 444

页数：8

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