Ca2+ signaling by plant Arabidopsis thaliana Pep peptides depends on AtPepR1, a receptor with guanylyl cyclase activity, and cGMP-activated Ca2+ channels

被引:179
作者
Qi, Zhi [1 ,2 ]
Verma, Rajeev [1 ]
Gehring, Chris [3 ]
Yamaguchi, Yube [4 ,5 ]
Zhao, Yichen [1 ]
Ryan, Clarence A. [5 ]
Berkowitz, Gerald A. [1 ]
机构
[1] Univ Connecticut, Dept Plant Sci, Agr Biotechnol Lab, Storrs, CT 06269 USA
[2] Inner Mongolia Univ, Coll Life Sci, Hohhot 010021, Inner Mongolia, Peoples R China
[3] King Abdullah Univ Sci & Technol, Computat Biosci Res Ctr, Thuwal 239556900, Saudi Arabia
[4] Hokkaido Univ, Grad Sch Agr, Crop Physiol Lab, Sapporo, Hokkaido 0608589, Japan
[5] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA
基金
美国国家科学基金会;
关键词
calcium signaling; plant innate immunity; flagellin receptor 2; brassinosteroid associated kinase 1; PATTERN-RECOGNITION RECEPTORS; GATED CATION CHANNEL; INNATE IMMUNE-RESPONSE; ION-CHANNEL; INOSITOL 1,4,5-TRISPHOSPHATE; ADENYLYL-CYCLASE; PLASMA-MEMBRANE; CELL-DEATH; CALCIUM; PERCEPTION;
D O I
10.1073/pnas.1000191107
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A family of peptide signaling molecules (AtPeps) and their plasma membrane receptor AtPepR1 are known to act in pathogendefense signaling cascades in plants. Little is currently known about the molecular mechanisms that link these signaling peptides and their receptor, a leucine-rich repeat receptor-like kinase, to downstream pathogen-defense responses. We identify some cellular activities of these molecules that provide the context for a model for their action in signaling cascades. AtPeps activate plasma membrane inwardly conducting Ca2+ permeable channels in mesophyll cells, resulting in cytosolic Ca2+ elevation. This activity is dependent on their receptor as well as a cyclic nucleotide-gated channel (CNGC2). We also show that the leucine-rich repeat receptor-like kinase receptor AtPepR1 has guanylyl cyclase activity, generating cGMP from GTP, and that cGMP can activate CNGC2-dependent cytosolic Ca2+ elevation. AtPep-dependent expression of pathogen-defense genes (PDF1.2, MPK3, and WRKY33) is mediated by the Ca2+ signaling pathway associated with AtPep peptides and their receptor. The work presented here indicates that extracellular AtPeps, which can act as danger-associated molecular patterns, signal by interaction with their receptor, AtPepR1, a plasma membrane protein that can generate cGMP. Downstream from AtPep and AtPepR1 in a signaling cascade, the cGMP-activated channel CNGC2 is involved in AtPep- and AtPepR1-dependent inward Ca2+ conductance and resulting cytosolic Ca2+ elevation. The signaling cascade initiated by AtPeps leads to expression of pathogen-defense genes in a Ca2+-dependent manner.
引用
收藏
页码:21193 / 21198
页数:6
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