Development of a high-throughput screen for protein catalysts: Application to the directed evolution of antibody aldolases

被引：6

作者：

Gildersleeve, J

Varvak, A

Atwell, S

Evans, D

Schultz, PG ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Novartis Res Fdn, Genom Inst, San Diego, CA 92121 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 48期

关键词：

aldol reaction; antibodies; directed evolution; high-throughput screening; proteins;

D O I：

10.1002/anie.200352117

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A semiautomated high-throughput system has been developed to express and purify proteins and assay their catalytic activity. The screen can be used to evolve activity, selectivity, and expression levels of proteins directly or in combination with selections. To illustrate its potential, the system was applied to the directed evolution of catalytic antibodies with aldolase activity.

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收藏

页码：5971 / 5973

页数：3

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