Human mtDNA sublimons resemble rearranged mitochondrial genoms found in pathological states

被引:114
作者
Kajander, OA
Rovio, AT
Majamaa, K
Poulton, J
Spelbrink, JN
Holt, IJ
Karhunen, PJ
Jacobs, HT [1 ]
机构
[1] Univ Tampere, Inst Med Technol, Tampere 33014, Finland
[2] Univ Tampere, Dept Forens Med, Tampere 33014, Finland
[3] Tampere Univ Hosp, Tampere, Finland
[4] Univ Oulu, Dept Neurol, Oulu, Finland
[5] Univ Oxford, Dept Paediat, Oxford, England
[6] MRC, Dunn Human Nutr Unit, Cambridge CB4 1XJ, England
[7] Univ Glasgow, Inst Biomed & Life Sci, Glasgow G12 8QQ, Lanark, Scotland
基金
英国医学研究理事会;
关键词
D O I
10.1093/hmg/9.19.2821
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Sublimons, originally identified in plant mitochondria, are defined as rearranged mtDNA molecules present at very low levels. We have analysed the primary structures of sublimons found in human cells and tissues and estimated their abundance. Each tissue of a given individual contains a wide range of different sublimons and the most abundant species differ between tissues in a substantially systematic manner, Sublimons are undetectable in rho (0) cells, indicating that they are bona fide derivatives of mtDNA, They are most prominent in postmitotic tissue subject to oxidative stress. Rearrangement break-points, often defined by short direct repeats, sire scattered, but hotspot regions are clearly identifiable, notably near the end of the D-loop, The region between the replication origins is therefore frequently eliminated, One other hotspot region is located adjacent to a known site of protein binding, suggesting that recombination may be facilitated by protein-protein interactions. For a given primary rearrangement, both deleted and partially duplicated species can be detected. Although each sublimon is typically present at a low level, at most a few copies per cell, sublimon abundance in a given tissue can vary over three orders of magnitude between healthy individuals. Collectively, therefore, they can represent a non-negligible fraction of total mtDNA, Their structures are very similar to those of the rearranged molecules found in pathological states, such as adPEO and MNGIE; therefore, we propose that, as in plants, human mtDNA sublimons represent a pool of variant molecules that can become amplified under pathological conditions, thus contributing to cellular dysfunction.
引用
收藏
页码:2821 / 2835
页数:15
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