Determination of flavonoids in Hippophae rhamnoides L. and its phytopharmaceuticals by capillary electrophoresis with electrochemical detection

被引：35

作者：

Chu, QC

Qu, WQ

Peng, YY

Cao, QH

Ye, JN ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

[2] E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China

来源：

CHROMATOGRAPHIA | 2003年 / 58卷 / 1-2期

关键词：

capillary electrophoresis; electrochemical detection; Hippophae rhamnoides L; flavonoids;

D O I：

10.1365/s10337-003-0011-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance capillary electrophoresis with electrochemical detection (CE-ED) method has been developed for the determination of the pharmacologically active flavonoids in Hippophae rhamnoides L. and its extract phytopharmaceuticals in this work. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential and the injection time were investigated to find the optimum conditions. Detection limits (S/N=3) ranged from 1.3 x 10(-7) to 5.9 x 10(-7) mg L-1 for all five analytes. This method was successfully used in the analysis of Hippophae rhamnoicles L. and its phytopharmaceuticals after a relatively simple extraction procedure, and the assay results were satisfactory.

引用

页码：67 / 71

页数：5

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