Purification and structural characterization of an α-glucosidase inhibitory polysaccharide from apricot (Armeniaca sibirica L. Lam.) pulp

被引：73

作者：

Cui, Jie ^{[1
]}

Gu, Xin ^{[1
]}

Wang, Fengjun ^{[2
]}

Ouyang, Jie ^{[2
]}

Wang, Jianzhong ^{[2
]}

机构：

[1] Beijing Forestry Univ, Coll Nat Conservat, Beijing 100083, Peoples R China

[2] Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2015年 / 121卷

关键词：

Apricot polysaccharide; alpha-Glucosidase inhibitor; Structural analysis; FRUITING BODIES;

D O I：

10.1016/j.carbpol.2014.12.065

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

In this study, the crude polysaccharide (APPS) from the fruiting bodies of apricot (Armeniaca sibirica L. Lam.) was isolated and fractionated by ultrafiltration and Sephadex G-75 gel chromatography. The hypoglycemic activities of all fractions were determined by a-glucosidase inhibitory activity in vitro. The fraction APPS1-2 showed the best activity with an IC50 of 6.06 mg/mL. The properties and chemical compositions of this fraction were analyzed with high-performance liquid chromatography, gel permeation chromatography-eighteen angle laser light scattering instrument, UV spectroscopy, infrared spectroscopy, and NMR spectroscopy (H-1). The results demonstrated that APPS1-2 was a neutral glycoconjugate with a molecular weight of 25.93 kDa. It comprised rhamnose, glucose, mannose, and galactose, with a relative molar ratio of 1.34:2.01:0.48:0.35. The backbone of APPS1-2 may consist of rhamnose and glucose, but its branches may consist of mannose and galactose. The IR and UV spectrum of APPS1-2 revealed the typical characteristics of heteropolysaccharide. 1H NMR spectrum showed that APPS1-2 contained a-configurations. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：309 / 314

页数：6

共 33 条

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Structural characterization and antioxidant activity of an extracellular polysaccharide isolated from Brevibacterium otitidis BTS 44 [J].