Efficient propagation of protocorm-like bodies of Phalaenopsis in liquid medium

Propagation by multiplication of protocorm-like bodies (PLB) of Phalaenopsis in liquid culture was investigated. VW-liquid medium was the most suitable for PLB multiplication, which showed 30% PLB multiplication. A cotton plate was chosen as a reactor for PLB propagation. The PLB in VW-liquid medium multiplied 80% with non-adsorbent cotton as a PLB support, as opposed to 70% in VW-solid medium; the PLB growth rate in that medium was much higher than that in the solid culture. When the cotton thickness was 6.0 mm that was corresponded to four layers of absorbent cotton and medium content containing the cotton was 7.2 mi g(-1) cotton, the PLB multiplication ratio was 100%; the most effective for the propagation of PLB. This indicates that there is an optimum wet condition in the liquid culture for an efficient PLB propagation.