Nucleolin is regulated both at the level of transcription and translation

被引：38

作者：

Bicknell, K

Brooks, G

Kaiser, P

Chen, HY

Dove, BK

Hiscox, JA ^{[1
]}

机构：

[1] Univ Leeds, Sch Biochem & Microbiol, Leeds, W Yorkshire, England

[2] Univ Reading, Sch Pharm, Cardiovasc Res Grp, Reading, Berks, England

[3] Inst Anim Hlth, Compton, England

[4] Univ Reading, Sch Anim & Microbial Sci, Virol Grp, Reading RG6 2AJ, Berks, England

[5] Astburg Ctr Struct Mol Biol, Leeds, W Yorkshire, England

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 332卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

developrnent; cardiomyocyte; heart; hyperplasia; hypertrophy; nucleolin; nucleolus; rat; TaqMan;

D O I：

10.1016/j.bbrc.2005.05.022

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nucleolin is a multi-functional protein that is located to the nucleolus. In tissue Culture cells, the stability of nucleolin is related to the proliferation status of the cell. During development, rat cardiomyocytes proliferate actively with increases in the mass of the heart being due to both hyperplasia and hypertrophy. The timing of this shift in the phenotype of the myocyte from one capable of undergoing hyperplasia to one that can grow only by hypertrophy occurs within 4 days of post-natal development. Thus, cardiomyocytes are an ideal model system in which to study the regulation of nucleolin during growth in vivo. Using Western blot and quantitative RT-PCR (TaqMan) we found that the amount of nucleolin is regulated both at the level of transcription and translation during the development of the cardiomyocyte. However, in cells which had exited the cell cycle and were subsequently given a hypertrophic stimulus, nucleolin was regulated post-transcriptionally. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：817 / 822

页数：6

共 33 条

[1] Selective increases in cytokine expression in the rat brain in response to striatal injection of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate and interleukin-1 [J].