Localization of a baculovirus-induced chitinase in the insect cell endoplasmic reticulum

被引：61

作者：

Thomas, CJ

Brown, HL

Hawes, CR

Lee, BY

Min, MK

King, LA

Possee, RD

机构：

[1] NERC, Inst Virol & Environm Microbiol, Oxford OX1 3SR, England

[2] Oxford Brookes Univ, Sch Biol Sci, Oxford OX3 0BP, England

[3] MOGAM Biotechnol Res Inst, Yongin Kun 449910, Kyonggi Do, South Korea

来源：

JOURNAL OF VIROLOGY | 1998年 / 72卷 / 12期

关键词：

D O I：

10.1128/JVI.72.12.10207-10212.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Confocal immunofluorescence microscopy was used to demonstrate that the Autographa californica nucleopolyhedrovirus (AcMNPV) chitinase was localized within the endoplasmic reticulum (ER) of virus-infected insect cells. This was consistent with removal of the signal peptide from the chitinase and an ER localization motif (KDEL) at the carboxyl end of the protein. Chitinase release from cells, a prerequisite for liquefaction of virus-infected insect larvae, appears to be aided by synthesis of the p10 protein. Deletion of p10 from the AcMNPV genome delayed the appearance of chitinase activity in the medium of virus-infected cells by 24 h and also delayed liquefaction of virus-infected Trichoplusia ni larvae by the same period.

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页码：10207 / 10212

页数：6

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共 22 条

[1] The sequence of the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus genome [J].

Ahrens, CH ;

Russell, RLQ ;

Funk, CJ ;

Evans, JT ;

Harwood, SH ;

Rohrmann, GF .

VIROLOGY, 1997, 229 (02) :381-399

[2] THE COMPLETE DNA-SEQUENCE OF AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS [J].

AYRES, MD ;

HOWARD, SC ;

KUZIO, J ;

LOPEZFERBER, M ;

POSSEE, RD .

VIROLOGY, 1994, 202 (02) :586-605

[3] Infection mechanisms of Botrytis species: Pre-penetration and pre-infection processes of dry and wet conidia [J].

Cole, L ;

Dewey, FM ;

Hawes, CR .

MYCOLOGICAL RESEARCH, 1996, 100 :277-286

[4] RECYCLING OF PROTEINS FROM THE GOLGI COMPARTMENT TO THE ER IN YEAST [J].

DEAN, N ;

PELHAM, HRB .

JOURNAL OF CELL BIOLOGY, 1990, 111 (02) :369-377

[5] PLANT AND MAMMALIAN SORTING SIGNALS FOR PROTEIN RETENTION IN THE ENDOPLASMIC-RETICULUM CONTAIN A CONSERVED EPITOPE [J].

DENECKE, J ;

DERYCKE, R ;

BOTTERMAN, J .

EMBO JOURNAL, 1992, 11 (06) :2345-2355

[6] Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genes [J].

Hawtin, RE ;

Zarkowska, T ;

Arnold, K ;

Thomas, CJ ;

Gooday, GW ;

King, LA ;

Kuzio, JA ;

Possee, RD .

VIROLOGY, 1997, 238 (02) :243-253

[7] IDENTIFICATION AND PRELIMINARY CHARACTERIZATION OF A CHITINASE GENE IN THE AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS-VIRUS GENOME [J].

HAWTIN, RE ;

ARNOLD, K ;

AYRES, MD ;

ZANOTTO, PMD ;

HOWARD, SC ;

GOODAY, GW ;

CHAPPELL, LH ;

KITTS, PA ;

KING, LA ;

POSSEE, RD .

VIROLOGY, 1995, 212 (02) :673-685

[8] Genetically variable triplet repeats in a RING-finger ORF of Helicoverpa species baculoviruses [J].

Le, TH ;

Wu, TQ ;

Robertson, A ;

Bulach, D ;

Cowan, P ;

Goodge, K ;

Tribe, D .

VIRUS RESEARCH, 1997, 49 (01) :67-77

[9] AUTHENTIC PROCESSING AND TARGETING OF ACTIVE MAIZE AUXIN-BINDING PROTEIN IN THE BACULOVIRUS EXPRESSION SYSTEM [J].

MACDONALD, H ;

HENDERSON, J ;

NAPIER, RM ;

VENIS, MA ;

HAWES, C ;

LAZARUS, CM .

PLANT PHYSIOLOGY, 1994, 105 (04) :1049-1057

[10] A C-TERMINAL SIGNAL PREVENTS SECRETION OF LUMINAL ER PROTEINS [J].

MUNRO, S ;

PELHAM, HRB .

CELL, 1987, 48 (05) :899-907