Cloning and characterization of mouse homolog of the CXC chemokine receptor CXCR1

被引：55

作者：

Fu, WX ^{[1
]}

Zhang, Y ^{[1
]}

Zhang, J ^{[1
]}

Chen, WF ^{[1
]}

机构：

[1] Peking Univ, Ctr Hlth, Dept Immunol, Beijing 100083, Peoples R China

来源：

CYTOKINE | 2005年 / 31卷 / 01期

基金：

中国国家自然科学基金;

关键词：

chemokine; chemokine receptor; inflammation; mouse CXCR1; neutrophils;

D O I：

10.1016/j.cyto.2005.02.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CXCR2/IL-8RB was the only receptor previously reported in mice for ELR+ CXC chemokines, whereas the receptors for these chemokines in human include both CXCR1 and CXCR2. In this study, we cloned the full length cDNA of the mouse CXCR1 (mCXCR1) gene. The deduced amino acid of mCXCR1 was 77% and 58% identical to the rat and human CXCR1, respectively. RT-PCR and Northern blot analysis showed that mCXCR1 mRNA was expressed in lung, spleen, thymus, peripheral blood leukocytes, as well as in the isolated neutrophils. In a mouse respiratory inflammation model induced by lipopolysaccharide, a large number of neutrophils infiltrated into the lung and, meanwhile, the mCXCR1 expression was significantly increased in the recruited neutrophils, suggesting that mCXCR1 may mediate the recruitment of neutrophils to the inflammation site under certain infections. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：9 / 17

页数：9

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