Biosynthetically lipid-modified human scFv fragments from phage display libraries as targeting molecules for immunoliposomes

被引：25

作者：

deKruif, J ^{[1
]}

Storm, G ^{[1
]}

vanBloois, L ^{[1
]}

Logtenberg, T ^{[1
]}

机构：

[1] UNIV UTRECHT,UTRECHT INST PHARMACEUT SCI,DEPT PHARMACEUT,NL-3508 TB UTRECHT,NETHERLANDS

来源：

FEBS LETTERS | 1996年 / 399卷 / 03期

关键词：

phage display; lipid-modified scFv; immunoliposome;

D O I：

10.1016/S0014-5793(96)01335-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A human anti-CD22 single chain (sc) Fv antibody fragment from a synthetic phage antibody display library was biosynthetically lipid-tagged by using Escherichia coli lipoprotein sequences. The purified anti-CDZ2 scFv lipoprotein was incorporated into liposomes by detergent dilution. Anti-CD22 immunoliposomes were shown to bind specifically in a dose- and time-dependent manner to CD22+ cell lines and CD22+ B-lymphocytes present in freshly isolated samples of blood mononuclear cells. The immunoliposomes were demonstrated to accumulate in intracellular compartments. Biosynthetically lipid-tagged human scFv antibody fragments isolated from phage display libraries may facilitate the construction of immunoliposomes with improved properties.

引用

页码：232 / 236

页数：5

共 16 条

[1] TARGETING OF ANTI-THY-1.1 MONOCLONAL-ANTIBODY CONJUGATED LIPOSOMES IN THY-1.1 MICE AFTER INTRAVENOUS ADMINISTRATION [J].

DEBS, RJ ;

HEATH, TD ;

PAPAHADJOPOULOS, D .

BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 901 (02) :183-190

[2] SELECTION AND APPLICATION OF HUMAN SINGLE-CHAIN FV ANTIBODY FRAGMENTS FROM A SEMISYNTHETIC PHAGE ANTIBODY DISPLAY LIBRARY WITH DESIGNED CDR3 REGIONS [J].

DEKRUIF, J ;

BOEL, E ;

LOGTENBERG, T .

JOURNAL OF MOLECULAR BIOLOGY, 1995, 248 (01) :97-105

[3] RAPID SELECTION OF CELL SUBPOPULATION-SPECIFIC HUMAN MONOCLONAL-ANTIBODIES FROM A SYNTHETIC PHAGE ANTIBODY LIBRARY [J].

DEKRUIF, J ;

TERSTAPPEN, L ;

BOEL, E ;

LOGTENBERG, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (09) :3938-3942

[4]

Fiske CH, 1925, J BIOL CHEM, V66, P375

[5] TRANSPORT AND ANCHORING OF BETA-LACTAMASE TO THE EXTERNAL SURFACE OF ESCHERICHIA-COLI [J].

FRANCISCO, JA ;

EARHART, CF ;

GEORGIOU, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (07) :2713-2717

[6] APPLICATIONS OF MONOCLONAL-ANTIBODIES IN CLINICAL ONCOLOGY [J].

GEORGE, AJT ;

SPOONER, RA ;

EPENETOS, AA .

IMMUNOLOGY TODAY, 1994, 15 (12) :559-561

[7] SYNEMIN AND VIMENTIN ARE COMPONENTS OF INTERMEDIATE FILAMENTS IN AVIAN ERYTHROCYTES [J].

GRANGER, BL ;

REPASKY, EA ;

LAZARIDES, E .

JOURNAL OF CELL BIOLOGY, 1982, 92 (02) :299-312

[8] Engineering liposomes for drug delivery: Progress and problems [J].

Gregoriadis, G .

TRENDS IN BIOTECHNOLOGY, 1995, 13 (12) :527-537

[9] MULTISUBUNIT PROTEINS ON THE SURFACE OF FILAMENTOUS PHAGE - METHODOLOGIES FOR DISPLAYING ANTIBODY (FAB) HEAVY AND LIGHT-CHAINS [J].

HOOGENBOOM, HR ;

GRIFFITHS, AD ;

JOHNSON, KS ;

CHISWELL, DJ ;

HUDSON, P ;

WINTER, G .

NUCLEIC ACIDS RESEARCH, 1991, 19 (15) :4133-4137

[10] LIPID-TAGGED ANTIBODIES - BACTERIAL EXPRESSION AND CHARACTERIZATION OF A LIPOPROTEIN SINGLE-CHAIN ANTIBODY FUSION PROTEIN [J].

LAUKKANEN, ML ;

TEERI, TT ;

KEINANEN, K .

PROTEIN ENGINEERING, 1993, 6 (04) :449-454

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