Molecular cloning and sequence determination of the complete genome of the virulent echovirus 9 strain Barty

被引：40

作者：

Zimmermann, H ^{[1
]}

Eggers, HJ ^{[1
]}

NelsenSalz, B ^{[1
]}

机构：

[1] UNIV COLOGNE,INST VIROL,D-50935 COLOGNE,GERMANY

来源：

VIRUS GENES | 1996年 / 12卷 / 02期

关键词：

echovirus; 9; genomic sequence; RGD-motif; pathogenicity;

D O I：

10.1007/BF00572953

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

As part of a study of the molecular basis of pathogenicity of echovirus 9, the complete nucleotide sequence of the mouse-virulent echovirus 9 strain Barty was determined. Excluding the poly(A) tail, the complete RNA genome is composed of 7451 bases. The postulated open reading frame extends from nucleotide (nt) 741 to 7349 and predicts a polyprotein of 2203 amino acids (aa). As compared with the sequence of the echovirus 9 prototype strain Hill, which is apathogenic for newborn mice, 1492 nt are exchanged, leading to 9% divergence of the deduced amino acid sequence. The foremost difference between both strains is located at the C-terminus of the capsid protein VP1. In the case of strain Barty, an additional 10 aa fragment, including an RGD motif, is inserted.

引用

页码：149 / 154

页数：6

共 13 条

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