Recognition of aminoglycoside antibiotics by enterococcal-staphylococcal aminoglycoside 3'-phosphotransferase type IIIa: Role of substrate amino groups

被引：27

作者：

McKay, GA

Roestamadji, J

Mobashery, S

Wright, GD

机构：

[1] MCMASTER UNIV,DEPT BIOCHEM,HAMILTON,ON L8N 3Z5,CANADA

[2] WAYNE STATE UNIV,DEPT CHEM,DETROIT,MI 48202

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1996年 / 40卷 / 11期

关键词：

D O I：

10.1128/AAC.40.11.2648

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The interactions of the aminoglycoside 3'-phosphotransferase IIIa with aminoglycoside antibiotics lacking specific amino groups were examined by steady-state kinetic analyses. The results demonstrate that an amino group on C-1 and either an amino or a hydroxyl group at the 2' and 6' positions are important for detoxification of aminoglycosides by this enzyme.

引用

收藏

页码：2648 / 2650

页数：3

相关论文

共 14 条

[1] N-15 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY OF NEOMYCIN-B AND RELATED AMINOGLYCOSIDES [J].

BOTTO, RE ;

COXON, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1983, 105 (04) :1021-1028

[2] Arrangement of substrates at the active site of an aminoglycoside antibiotic 3'-phosphotransferase as determined by NMR [J].

Cox, JR ;

McKay, GA ;

Wright, GD ;

Serpersu, EH .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (06) :1295-1301

[3]

DAVIES JE, 1991, ANTIBIOTICS LAB MED, P691

[4] THE AMINOGLYCOSIDES [J].

EDSON, RS ;

TERRELL, CL .

MAYO CLINIC PROCEEDINGS, 1991, 66 (11) :1158-1164

[5] BROAD-SPECTRUM AMINOGLYCOSIDE PHOSPHOTRANSFERASE TYPE-III FROM ENTEROCOCCUS - OVEREXPRESSION, PURIFICATION, AND SUBSTRATE-SPECIFICITY [J].

MCKAY, GA ;

THOMPSON, PR ;

WRIGHT, GD .

BIOCHEMISTRY, 1994, 33 (22) :6936-6944

[6] KINETIC MECHANISM OF AMINOGLYCOSIDE PHOSPHOTRANSFERASE TYPE IIIA - EVIDENCE FOR A THEORELL-CHANCE MECHANISM [J].

MCKAY, GA ;

WRIGHT, GD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (42) :24686-24692

[7] PENICILLIN-TOBRAMYCIN SYNERGISM AGAINST ENTEROCOCCI - COMPARISON WITH PENICILLIN AND GENTAMICIN [J].

MOELLERING, RC ;

WENNERSTEN, C ;

WEINSTEIN, AJ .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1973, 3 (04) :526-529

[8] THE LIFE AND TIMES OF THE ENTEROCOCCUS [J].

MURRAY, BE .

CLINICAL MICROBIOLOGY REVIEWS, 1990, 3 (01) :46-65

[9] LOSS OF INDIVIDUAL ELECTROSTATIC INTERACTIONS BETWEEN AMINOGLYCOSIDE ANTIBIOTICS AND RESISTANCE ENZYMES AS AN EFFECTIVE MEANS TO OVERCOMING BACTERIAL DRUG-RESISTANCE [J].

ROESTAMADJI, J ;

GRAPSAS, I ;

MOBASHERY, S .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (45) :11060-11069

[10] MOLECULAR-GENETICS OF AMINOGLYCOSIDE RESISTANCE GENES AND FAMILIAL RELATIONSHIPS OF THE AMINOGLYCOSIDE-MODIFYING ENZYMES [J].

SHAW, KJ ;

RATHER, PN ;

HARE, RS ;

MILLER, GH .

MICROBIOLOGICAL REVIEWS, 1993, 57 (01) :138-163