Development of a rapid diagnostic assay for methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative Staphylococcus

被引：20

作者：

Lindsey, Wesley C. ^{[1
]}

Woodruff, Evelyn S. ^{[1
]}

Weed, Diane ^{[2
]}

Ward, David C. ^{[3
]}

Jenison, Robert D. ^{[1
]}

机构：

[1] Inverness Med Innovat Inc, Louisville, CO 80027 USA

[2] Denver Hlth Med Ctr, Dept Microbiol, Denver, CO 80204 USA

[3] Nevada Canc Inst, Las Vegas, NV 89135 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2008年 / 61卷 / 03期

关键词：

MRSA; MSSA; thin-film biosensor chip; mecA; femB; CoNS;

D O I：

10.1016/j.diagmicrobio.2008.03.002

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We describe here a rapid assay for the detection of the tuf gene for the identification of Staphylococcus genus, the femB gene for the identification of Staphylococcus aureus species, and the mecA gene for the identification of methicillin resistance directly from BACTEC blood culture bottles showing Gram-positive cocci in clusters. The test, configured on a thin-film biosensor platform, allows for detection of genomic DNA from blood culture samples without the need for nucleic acid amplification. In an initial study to validate the technology, 107 consecutive positive blood cultures were tested on the thin-film biosensor, and the assay exhibited 100% concordance in comparison with standard microbiological methods for identifying methicillin-susceptible and methicillin-resistant S. aureus and for identifying methicillin-susceptible and methicillin-resistant coagulase-negative Staphylococcus. Results were obtained within 90 min directly from signal positive bottles with no instrumentation required. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：273 / 279

页数：7

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