The Influenza A Virus PB2, PA, NP, and M Segments Play a Pivotal Role during Genome Packaging

被引:62
作者
Gao, Qinshan [1 ]
Chou, Yi-Ying [1 ]
Doganay, Sultan [5 ]
Vafabakhsh, Reza [4 ]
Ha, Taekjip [3 ,4 ,5 ]
Palese, Peter [1 ,2 ]
机构
[1] Mt Sinai Sch Med, Dept Microbiol, New York, NY USA
[2] Mt Sinai Sch Med, Dept Med, New York, NY USA
[3] Univ Illinois, Howard Hughes Med Inst, Urbana, IL 61801 USA
[4] Univ Illinois, Dept Phys, Urbana, IL 61801 USA
[5] Univ Illinois, Ctr Biophys & Computat Biol, Urbana, IL 61801 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
VIRAL-RNA; RIBONUCLEOPROTEIN COMPLEXES; SIGNALS; PROTEIN; TRANSPORT; REGIONS;
D O I
10.1128/JVI.00662-12
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genomes of influenza A viruses consist of eight negative-strand RNA segments. Recent studies suggest that influenza viruses are able to specifically package their segmented genomes into the progeny virions. Segment-specific packaging signals of influenza virus RNAs (vRNAs) are located in the 5' and 3' noncoding regions, as well as in the terminal regions, of the open reading frames. How these packaging signals function during genome packaging remains unclear. Previously, we generated a 7-segmented virus in which the hemagglutinin (HA) and neuraminidase (NA) segments of the influenza A/Puerto Rico/8/34 virus were replaced by a chimeric influenza C virus hemagglutinin/esterase/fusion (HEF) segment carrying the HA packaging sequences. The robust growth of the HEF virus suggested that the NA segment is not required for the packaging of other segments. In this study, in order to determine the roles of the other seven segments during influenza A virus genome assembly, we continued to use this HEF virus as a tool and analyzed the effects of replacing the packaging sequences of other segments with those of the NA segment. Our results showed that deleting the packaging signals of the PB1, HA, or NS segment had no effect on the growth of the HEF virus, while growth was greatly impaired when the packaging sequence of the PB2, PA, nucleoprotein (NP), or matrix (M) segment was removed. These results indicate that the PB2, PA, NP, and M segments play a more important role than the remaining four vRNAs during the genome-packaging process.
引用
收藏
页码:7043 / 7051
页数:9
相关论文
共 34 条
[1]   A Rab11-and Microtubule-Dependent Mechanism for Cytoplasmic Transport of Influenza A Virus Viral RNA [J].
Amorim, Maria Joao ;
Bruce, Emily A. ;
Read, Eliot K. C. ;
Foeglein, Agnes ;
Mahen, Robert ;
Stuart, Amanda D. ;
Digard, Paul .
JOURNAL OF VIROLOGY, 2011, 85 (09) :4143-4156
[2]   One influenza virus particle packages eight unique viral RNAs as shown by FISH analysis [J].
Chou, Yi-ying ;
Vafabakhsh, Reza ;
Doganay, Sultan ;
Gao, Qinshan ;
Ha, Taekjip ;
Palese, Peter .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (23) :9101-9106
[3]   Defective RNAs inhibit the assembly of influenza virus genome segments in a segment-specific manner [J].
Duhaut, SD ;
McCauley, JW .
VIROLOGY, 1996, 216 (02) :326-337
[4]   Human Immunodeficiency Virus Rev-Binding Protein Is Essential for Influenza A Virus Replication and Promotes Genome Trafficking in Late-Stage Infection [J].
Eisfeld, Amie J. ;
Neumann, Gabriele ;
Kawaoka, Yoshihiro .
JOURNAL OF VIROLOGY, 2011, 85 (18) :9588-9598
[5]   RAB11A Is Essential for Transport of the Influenza Virus Genome to the Plasma Membrane [J].
Eisfeld, Amie J. ;
Kawakami, Eiryo ;
Watanabe, Tokiko ;
Neumann, Gabriele ;
Kawaoka, Yoshihiro .
JOURNAL OF VIROLOGY, 2011, 85 (13) :6117-6126
[6]   Rescue of influenza A virus from recombinant DNA [J].
Fodor, E ;
Devenish, L ;
Engelhardt, OG ;
Palese, P ;
Brownlee, GG ;
García-Sastre, A .
JOURNAL OF VIROLOGY, 1999, 73 (11) :9679-9682
[7]   A supramolecular assembly formed by influenza A virus genomic RNA segments [J].
Fournier, Emilie ;
Moules, Vincent ;
Essere, Boris ;
Paillart, Jean-Christophe ;
Sirbat, Jean-Daniel ;
Isel, Catherine ;
Cavalier, Annie ;
Rolland, Jean-Paul ;
Thomas, Daniel ;
Lina, Bruno ;
Marquet, Roland .
NUCLEIC ACIDS RESEARCH, 2012, 40 (05) :2197-2209
[8]   Importance of both the coding and the segment-specific noncoding regions of the influenza a virus NS segment for its efficient incorporation into virions [J].
Fujii, K ;
Fujii, Y ;
Noda, T ;
Muramoto, Y ;
Watanabe, T ;
Takada, A ;
Goto, H ;
Horimoto, T ;
Kawaoka, Y .
JOURNAL OF VIROLOGY, 2005, 79 (06) :3766-3774
[9]   Selective incorporation of influenza virus RNA segments into virions [J].
Fujii, Y ;
Goto, H ;
Watanabe, T ;
Yoshida, T ;
Kawaoka, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (04) :2002-2007
[10]   A seven-segmented influenza A virus expressing the influenza C virus glycoprotein HEF [J].
Gao, Qinshan ;
Brydon, Edward W. A. ;
Palese, Peter .
JOURNAL OF VIROLOGY, 2008, 82 (13) :6419-6426