OsTIR1 and OsAFB2 Downregulation via OsmiR393 Overexpression Leads to More Tillers, Early Flowering and Less Tolerance to Salt and Drought in Rice

被引:264
作者
Xia, Kuaifei [1 ,2 ]
Wang, Ren [1 ,3 ]
Ou, Xiaojin [1 ,3 ]
Fang, Zhongming [1 ,3 ]
Tian, Changen [4 ]
Duan, Jun [1 ,2 ]
Wang, Yaqin [5 ]
Zhang, Mingyong [1 ,2 ]
机构
[1] Chinese Acad Sci, S China Bot Garden, Key Lab S China Agr Plant Genet & Breeding, Guangzhou, Guangdong, Peoples R China
[2] Chinese Acad Sci, S China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou, Guangdong, Peoples R China
[3] Chinese Acad Sci, Grad Univ, Guangzhou, Guangdong, Peoples R China
[4] Guangzhou Univ, Sch Life Sci, Guangzhou, Guangdong, Peoples R China
[5] S China Normal Univ, Guangdong Key Lab Biotechnol Plant Dev, Coll Life Sci, Guangzhou, Guangdong, Peoples R China
来源
PLOS ONE | 2012年 / 7卷 / 01期
关键词
PLANT MICRORNAS; SMALL RNAS; AUXIN; GENE; ARABIDOPSIS; TIME; STRESS; EXPRESSION; SALINITY; IDENTIFICATION;
D O I
10.1371/journal.pone.0030039
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The microRNA miR393 has been shown to play a role in plant development and in the stress response by targeting mRNAs that code for the auxin receptors in Arabidopsis. In this study, we verified that two rice auxin receptor gene homologs (OsTIR1 and OsAFB2) could be targeted by OsmiR393 (Os for Oryza sativa). Two new phenotypes (increased tillers and early flowering) and two previously observed phenotypes (reduced tolerance to salt and drought and hyposensitivity to auxin) were observed in the OsmiR393-overexpressing rice plants. The OsmiR393-overexpressing rice demonstrated hyposensitivity to synthetic auxin-analog treatments. These data indicated that the phenotypes of OsmiR393-overexpressing rice may be caused through hyposensitivity to the auxin signal by reduced expression of two auxin receptor genes (OsTIR1 and OsAFB2). The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering. The positive phenotypes (increased tillers and early flowering) and negative phenotypes (reduced tolerance to salt and hyposensitivity to auxin) of OsmiR393-overexpressing rice present a dilemma for molecular breeding.
引用
收藏
页码:364 / 373
页数:10
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