Inhibition of human cytomegalovirus replication by small interfering RNAs

被引：66

作者：

Wiebusch, L ^{[1
]}

Truss, M ^{[1
]}

Hagemeier, C ^{[1
]}

机构：

[1] Humboldt Univ, Mol Biol Lab, Dept Pediat, Berlin, Germany

来源：

JOURNAL OF GENERAL VIROLOGY | 2004年 / 85卷

关键词：

D O I：

10.1099/vir.0.19453-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Small interfering RNAs (siRNAs) are the mediators of a sequence-specific process of gene silencing called RNA interference (RNAi). Here, we show that synthetic siRNAs against essential gene products of human cytomegalovirus (HCMV) can trigger RNAi in serum-starved, infected primary fibroblasts, as well as in U373 cells, leading to effective inhibition of viral DNA replication. This opens new possibilities for antiviral strategies and for the analysis of viral and cellular genes important to HCMV physiology.

引用

页码：179 / 184

页数：6

共 25 条

[1] ANTIVIRAL ACTIVITY OF A PHOSPHOROTHIOATE OLIGONUCLEOTIDE COMPLEMENTARY TO RNA OF THE HUMAN CYTOMEGALOVIRUS MAJOR IMMEDIATE-EARLY REGION [J].

AZAD, RF ;

DRIVER, VB ;

TANAKA, K ;

CROOKE, RM ;

ANDERSON, KP .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (09) :1945-1954

[2] Human cytomegalovirus infection leads to accumulation of geminin and inhibition of the licensing of cellular DNA replication [J].

Biswas, N ;

Sanchez, V ;

Spector, DH .

JOURNAL OF VIROLOGY, 2003, 77 (04) :2369-2376

[3] Human cytomegalovirus inhibits cellular DNA synthesis and arrests productively infected cells in late G1 [J].

Bresnahan, WA ;

Boldogh, I ;

Thompson, EA ;

Albrecht, T .

VIROLOGY, 1996, 224 (01) :150-160

[4] Inhibition of HIV-1 infection by small interfering RNA-mediated RNA interference [J].

Capodici, J ;

Karikó, K ;

Weissman, D .

JOURNAL OF IMMUNOLOGY, 2002, 169 (09) :5196-5201

[5] Potent and specific inhibition of human immunodeficiency virus type 1 replication by RNA interference [J].

Coburn, GA ;

Cullen, BR .

JOURNAL OF VIROLOGY, 2002, 76 (18) :9225-9231

[6] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].

Elbashir, SM ;

Harborth, J ;

Lendeckel, W ;

Yalcin, A ;

Weber, K ;

Tuschl, T .

NATURE, 2001, 411 (6836) :494-498

[7] Analysis of gene function in somatic mammalian cells using small interfering RNAs [J].

Elbashir, SM ;

Harborth, J ;

Weber, K ;

Tuschl, T .

METHODS, 2002, 26 (02) :199-213

[8] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

[9] RNA interference of influenza virus production by directly targeting rnRNA for degradation and indirectly inhibiting all viral RNA transcription [J].

Ge, Q ;

McManus, MT ;

Nguyen, T ;

Shen, CH ;

Sharp, PA ;

Eisen, HN ;

Chen, JZ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (05) :2718-2723

[10] Short interfering RNA confers intracellular antiviral immunity in human cells [J].

Gitlin, L ;

Karelsky, S ;

Andino, R .

NATURE, 2002, 418 (6896) :430-434

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