A novel cytochrome P450 3A isoenzyme in rat intestinal microsomes

被引：21

作者：

Gushchin, GV ^{[1
]}

Gushchin, MI ^{[1
]}

Gerber, N ^{[1
]}

Boyd, RT ^{[1
]}

机构：

[1] Ohio State Univ, Coll Med, Dept Pharmacol, Columbus, OH 43210 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 255卷 / 02期

关键词：

D O I：

10.1006/bbrc.1999.0200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PCR with several pairs of primers facilitates screening for new isoenzymes among highly homologous cytochrome P450s (CYPs). Combinations of two pairs of primers, which amplify N- and C-terminal coding sequences of either CYP3A1/CYP3A23 or CYP3A2 detected the presence of a previously unrecognized CYP3A in enterocyte microsomes isolated from rats. PCR, Northern blot, and immunoblotting with specific antibodies indicated that this isoenzyme is clearly distinguishable from CYP3A1, 3A23 or 3A2. Sequencing of a 285 bp coding fragment of this gene revealed 97% similarity with rat olfactory CYP3A9 (P450olf3). (C) 1999 Academic Press.

引用

页码：394 / 398

页数：5

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