Canonical A-to-I and C-to-U RNA Editing Is Enriched at 3′UTRs and microRNA Target Sites in Multiple Mouse Tissues

被引：61

作者：

Gu, Tongjun ^{[1
]}

Buaas, Frank W. ^{[1
]}

Simons, Allen K. ^{[1
]}

Ackert-Bicknell, Cheryl L. ^{[1
]}

Braun, Robert E. ^{[1
]}

Hibbs, Matthew A. ^{[1
]}

机构：

[1] Jackson Lab, Bar Harbor, ME 04609 USA

来源：

PLOS ONE | 2012年 / 7卷 / 03期

基金：

美国国家卫生研究院;

关键词：

PARALLEL DNA; IDENTIFICATION; SEQUENCES; ERROR;

D O I：

10.1371/journal.pone.0033720

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

RNA editing is a process that modifies RNA nucleotides and changes the efficiency and fidelity of the central dogma. Enzymes that catalyze RNA editing are required for life, and defects in RNA editing are associated with many diseases. Recent advances in sequencing have enabled the genome-wide identification of RNA editing sites in mammalian transcriptomes. Here, we demonstrate that canonical RNA editing (A-to-I and C-to-U) occurs in liver, white adipose, and bone tissues of the laboratory mouse, and we show that apparent non-canonical editing (all other possible base substitutions) is an artifact of current high-throughput sequencing technology. Further, we report that high-confidence canonical RNA editing sites can cause non-synonymous amino acid changes and are significantly enriched in 3' UTRs, specifically at microRNA target sites, suggesting both regulatory and functional consequences for RNA editing.

引用

页数：7

共 28 条

[1] Accurate identification of A-to-I RNA editing in human by transcriptome sequencing [J].